| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ROBO2 (450-660) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ROBO2 |
| Gene ID: |
6092 |
| Uniprot ID: |
ROBO2_HUMAN |
| Immunogen Region: |
450-660 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 450-660 of human ROBO2 (NP_001122401.1). |
| Immunogen Sequence: |
TALLKCKATGDPLPVISWLK EGFTFPGRDPRATIQEQGTL QIKNLRISDTGTYTCVATSS SGETSWSAVLDVTESGATIS KNYDLSDLPGPPSKPQVTDV TKNSVTLSWQPGTPGTLPAS AYIIEAFSQSVSNSWQTVAN HVKTTLYTVRGLRPNTIYLF MVRAINPQGLSDPSPMSDPV RTQDISPPAQGVDHRQVQKE LGDVLVRLHNP |
| Function | Receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. |
| Protein Name | Roundabout Homolog 2 |
| Database Links | Reactome: R-HSA-376176Reactome: R-HSA-428542Reactome: R-HSA-8985801Reactome: R-HSA-9010553Reactome: R-HSA-9010642 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Roundabout Homolog 2 antibodyAnti-ROBO2 antibodyAnti-KIAA1568 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and ANKRD52 knockout (KO) 293T cells, using [KO Validated] ANKRD52 Rabbit polyclonal antibody (STJ11100834) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89769/358241/STJ11100834_1__55275.1713122574.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ANKRD52 knockout (KO) 293T cells, using [KO Validated] ANKRD52 Rabbit polyclonal antibody (STJ11100834) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
