| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RNF6 |
| Gene ID: | 6049 |
| Uniprot ID: | RNF6_HUMAN |
| Immunogen Region: | 1-300 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RNF6 (NP_898864.1). |
| Immunogen Sequence: | MNQSRSRSDGGSEETLPQDH NHHENERRWQQERLHREEAY YQFINELNDEDYRLMRDHNL LGTPGEITSEELQQRLDGVK EQLASQPDLRDGTNYRDSEV PRESSHEDSLLEWLNTFRRT GNATRSGQNGNQTWRAVSRT NPNNGEFRFSLEIHVNHENR GFEIHGEDYTDIPLSDSNRD HTANRQQRSTSPVARRTRSQ TSVNFNGSSSNIPRTRLASR GQNPAEGSFSTLGRLRNGI |
| Tissue Specificity | Weakly expressed in peripheral blood, spleen, prostate, testis and ovary. According to a report, it is preferentially expressed in testis and ovary and hardly detected in other tissues. |
| Function | E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation. Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover. Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity. May also bind DNA and function as a transcriptional regulator. Mediates polyubiquitination of QKI in macrophages, leading to its degradation. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf6 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | NucleusCytoplasmCell ProjectionAxonPml BodyLocalizes To The Pml Nuclear Bodies In Sertoli Cells |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf6 antibodyAnti-RNF6 antibodyAnti-SPG2 antibody |
Information sourced from Uniprot.org