| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RNF6 (1-300) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RNF6 |
| Gene ID: |
6049 |
| Uniprot ID: |
RNF6_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RNF6 (NP_898864.1). |
| Immunogen Sequence: |
MNQSRSRSDGGSEETLPQDH NHHENERRWQQERLHREEAY YQFINELNDEDYRLMRDHNL LGTPGEITSEELQQRLDGVK EQLASQPDLRDGTNYRDSEV PRESSHEDSLLEWLNTFRRT GNATRSGQNGNQTWRAVSRT NPNNGEFRFSLEIHVNHENR GFEIHGEDYTDIPLSDSNRD HTANRQQRSTSPVARRTRSQ TSVNFNGSSSNIPRTRLASR GQNPAEGSFSTLGRLRNGI |
| Tissue Specificity | Weakly expressed in peripheral blood, spleen, prostate, testis and ovary. According to a report, it is preferentially expressed in testis and ovary and hardly detected in other tissues. |
| Function | E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation. Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover. Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity. May also bind DNA and function as a transcriptional regulator. Mediates polyubiquitination of QKI in macrophages, leading to its degradation. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf6 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | NucleusCytoplasmCell ProjectionAxonPml BodyLocalizes To The Pml Nuclear Bodies In Sertoli Cells |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf6 antibodyAnti-RNF6 antibodyAnti-SPG2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
