| Host: |
Rabbit |
| Applications: |
WB/IF/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RING1B/RNF2 (1-336) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200IP 1:50-1:200ChIP 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RNF2 |
| Gene ID: |
6045 |
| Uniprot ID: |
RING2_HUMAN |
| Immunogen Region: |
1-336 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human RING1B/RING1B/RNF2 (NP_009143.1). |
| Immunogen Sequence: |
MSQAVQTNGTQPLSKTWELS LYELQRTPQEAITDGLEIVV SPRSLHSELMCPICLDMLKN TMTTKECLHRFCADCIITAL RSGNKECPTCRKKLVSKRSL RPDPNFDALISKIYPSRDEY EAHQERVLARINKHNNQQAL SHSIEEGLKIQAMNRLQRGK KQQIENGSGAEDNGDSSHCS NASTHSNQEAGPSNKRTKTS DDSGLELDNNNAAMAIDPVM DGASEIELVFRPHPTLMEK |
| Post Translational Modifications | Monoubiquitinated, by auto-ubiquitination. Polyubiquitinated in the presence of UBE2D3 (in vitro). |
| Function | E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation. H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity (Probable). Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes. Also acts as a negative regulator of autophagy by mediating ubiquitination of AMBRA1, leading to its subsequent degradation. |
| Protein Name | E3 Ubiquitin-Protein Ligase Ring2Huntingtin-Interacting Protein 2-Interacting Protein 3Hip2-Interacting Protein 3Protein DingRing Finger Protein 1bRing1bRing Finger Protein 2Ring Finger Protein Bap-1Ring-Type E3 Ubiquitin Transferase Ring2 |
| Database Links | Reactome: R-HSA-2559580Reactome: R-HSA-3108214Reactome: R-HSA-3899300Reactome: R-HSA-4551638Reactome: R-HSA-4570464Reactome: R-HSA-4655427Reactome: R-HSA-8939243Reactome: R-HSA-8943724Reactome: R-HSA-8953750 |
| Cellular Localisation | NucleusCytoplasmChromosomeEnriched On Inactive X Chromosome (Xi) In Female Trophoblast Stem (Ts) Cells As Well As Differentiating Embryonic Stem (Es) CellsThe Enrichment On Xi Is Transient During Ts And Es Cell DifferentiationThe Association With Xi Is Mitotically Stable In Non-Differentiated Ts Cells |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Ring2 antibodyAnti-Huntingtin-Interacting Protein 2-Interacting Protein 3 antibodyAnti-Hip2-Interacting Protein 3 antibodyAnti-Protein Ding antibodyAnti-Ring Finger Protein 1b antibodyAnti-Ring1b antibodyAnti-Ring Finger Protein 2 antibodyAnti-Ring Finger Protein Bap-1 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Ring2 antibodyAnti-RNF2 antibodyAnti-BAP1 antibodyAnti-DING antibodyAnti-HIPI3 antibodyAnti-RING1B antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
