| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RNF111 (400-500) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RNF111 |
| Gene ID: |
54778 |
| Uniprot ID: |
RN111_HUMAN |
| Immunogen Region: |
400-500 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human RNF111 (NP_001257459.1). |
| Immunogen Sequence: |
ARMESQATSASINNSNPSTS EQASDTASAVTSSQPSTVSE TSATLTSNSTTGTSIGDDSR RTTSSAVTETGPPAMPRLPS CCPQHSPCGGSSQNHHALGH P |
| Tissue Specificity | Broadly expressed. |
| Function | E3 ubiquitin-protein ligase. Required for mesoderm patterning during embryonic development. Acts as an enhancer of the transcriptional responses of the SMAD2/SMAD3 effectors, which are activated downstream of BMP. Acts by mediating ubiquitination and degradation of SMAD inhibitors such as SMAD7, inducing their proteasomal degradation and thereby enhancing the transcriptional activity of TGF-beta and BMP. In addition to enhance transcription of SMAD2/SMAD3 effectors, also regulates their turnover by mediating their ubiquitination and subsequent degradation, coupling their activation with degradation, thereby ensuring that only effectors 'in use' are degraded. Activates SMAD3/SMAD4-dependent transcription by triggering signal-induced degradation of SNON isoform of SKIL. Associates with UBE2D2 as an E2 enzyme. Specifically binds polysumoylated chains via SUMO interaction motifs (SIMs) and mediates ubiquitination of sumoylated substrates. Catalyzes 'Lys-63'-linked ubiquitination of sumoylated XPC in response to UV irradiation, promoting nucleotide excision repair. Mediates ubiquitination and degradation of sumoylated PML. The regulation of the BMP-SMAD signaling is however independent of sumoylation and is not dependent of SUMO interaction motifs (SIMs). |
| Protein Name | E3 Ubiquitin-Protein Ligase ArkadiaRing Finger Protein 111Hrnf111Ring-Type E3 Ubiquitin Transferase Arkadia |
| Database Links | Reactome: R-HSA-2173795Reactome: R-HSA-2173796Reactome: R-HSA-5696395Reactome: R-HSA-983168 |
| Cellular Localisation | NucleusCytoplasmPml BodyUpon Tgf-Beta TreatmentTranslocates From Nucleus To Cytosol |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Arkadia antibodyAnti-Ring Finger Protein 111 antibodyAnti-Hrnf111 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Arkadia antibodyAnti-RNF111 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
