| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MINA (1-300) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RIOX2 |
| Gene ID: |
84864 |
| Uniprot ID: |
RIOX2_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RIOX2/RIOX2/MINA5353 (NP_694822.2). |
| Immunogen Sequence: |
MPKKAKPTGSGKEEGPAPCK QMKLEAAGGPSALNFDSPSS LFESLISPIKTETFFKEFWE QKPLLIQRDDPALATYYGSL FKLTDLKSLCSRGMYYGRDV NVCRCVNGKKKVLNKDGKAH FLQLRKDFDQKRATIQFHQP QRFKDELWRIQEKLECYFGS LVGSNVYITPAGSQGLPPHY DDVEVFILQLEGEKHWRLYH PTVPLAREYSVEAEERIGRP VHEFMLKPGDLLYFPRGTI |
| Tissue Specificity | Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines. |
| Function | Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. Also catalyzes the hydroxylation of 60S ribosomal protein L27a on 'His-39'. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles. |
| Protein Name | Ribosomal Oxygenase 260s Ribosomal Protein L27a Histidine HydroxylaseBifunctional Lysine-Specific Demethylase And Histidyl-Hydroxylase MinaHistone Lysine Demethylase MinaMyc-Induced Nuclear AntigenMineral Dust-Induced Gene ProteinNucleolar Protein 52Ribosomal Oxygenase MinaRox |
| Database Links | Reactome: R-HSA-3214842 |
| Cellular Localisation | NucleusNucleolus |
| Alternative Antibody Names | Anti-Ribosomal Oxygenase 2 antibodyAnti-60s Ribosomal Protein L27a Histidine Hydroxylase antibodyAnti-Bifunctional Lysine-Specific Demethylase And Histidyl-Hydroxylase Mina antibodyAnti-Histone Lysine Demethylase Mina antibodyAnti-Myc-Induced Nuclear Antigen antibodyAnti-Mineral Dust-Induced Gene Protein antibodyAnti-Nucleolar Protein 52 antibodyAnti-Ribosomal Oxygenase Mina antibodyAnti-Rox antibodyAnti-RIOX2 antibodyAnti-MDIG antibodyAnti-MINA antibodyAnti-MINA53 antibodyAnti-NO52 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")