Anti-REST antibody (593-643) (STJA0006421)

SKU:
STJA0006421

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RE1-silencing transcription factor (593-643) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: REST
Gene ID: 5978
Uniprot ID: REST_HUMAN
Immunogen Region: 593-643
Specificity: This antibody detects endogenous levels of human REST
Immunogen: Synthesized peptide derived from human REST AA range: 593-643
Post Translational Modifications O-glycosylated. Phosphorylated.phosphorylation is required for ubiquitination. Ubiquitinated.ubiquitination is mediated by BTRC and leads to proteasomal degradation in G2 phase. Ubiquitination increases during neuronal differentiation. Deubiquitinated by USP7.leading to its stabilization and promoting the maintenance of neural progenitor cells.
Function Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression. Represses the expression of SRRM4 in non-neural cells to prevent the activation of neural-specific splicing events and to prevent production of REST isoform 3. Repressor activity may be inhibited by forming heterodimers with isoform 3, thereby preventing binding to NRSE or binding to corepressors and leading to derepression of target genes. Also maintains repression of neuronal genes in neural stem cells, and allows transcription and differentiation into neurons by dissociation from RE1/NRSE sites of target genes. Thereby is involved in maintaining the quiescent state of adult neural stem cells and preventing premature differentiation into mature neurons. Plays a role in the developmental switch in synaptic NMDA receptor composition during postnatal development, by repressing GRIN2B expression and thereby altering NMDA receptor properties from containing primarily GRIN2B to primarily GRIN2A subunits. Acts as a regulator of osteoblast differentiation. Key repressor of gene expression in hypoxia.represses genes in hypoxia by direct binding to an RE1/NRSE site on their promoter regions. May also function in stress resistance in the brain during aging.possibly by regulating expression of genes involved in cell death and in the stress response. Repressor of gene expression in the hippocampus after ischemia by directly binding to RE1/NRSE sites and recruiting SIN3A and RCOR1 to promoters of target genes, thereby promoting changes in chromatin modifications and ischemia-induced cell death. After ischemia, might play a role in repression of miR-132 expression in hippocampal neurons, thereby leading to neuronal cell death. Negatively regulates the expression of SRRM3 in breast cancer cell lines. Isoform 3: Binds to the 3' region of the neuron-restrictive silencer element (NRSE), with lower affinity than full-length REST isoform 1. Exhibits weaker repressor activity compared to isoform 1. May negatively regulate the repressor activity of isoform 1 by binding to isoform 1, thereby preventing its binding to NRSE and leading to derepression of target genes. However, in another study, does not appear to be implicated in repressor activity of a NRSE motif-containing reporter construct nor in inhibitory activity on the isoform 1 transcriptional repressor activity. Post-transcriptional inactivation of REST by SRRM4-dependent alternative splicing into isoform 3 is required in mechanosensory hair cells in the inner ear for derepression of neuronal genes and hearing.
Protein Name Re1-Silencing Transcription Factor
Neural-Restrictive Silencer Factor
X2 Box Repressor
Database Links Reactome: R-HSA-3214815
Reactome: R-HSA-8943724
Reactome: R-HSA-9031628
Reactome: R-HSA-9679191
Reactome: R-HSA-9768777
Cellular Localisation Nucleus
Cytoplasm
Colocalizes With Zfp90 In The Nucleus
In Response To Hypoxia
There Is A More Pronounced Increase In Levels In The Nucleus As Compared To The Cytoplasm
In Aging Neurons
Increased Levels In The Nucleus As Compared To The Cytoplasm
Isoform 2: Cytoplasm
Isoform 3: Nucleus
Isoform 4: Cytoplasm
Alternative Antibody Names Anti-Re1-Silencing Transcription Factor antibody
Anti-Neural-Restrictive Silencer Factor antibody
Anti-X2 Box Repressor antibody
Anti-REST antibody
Anti-NRSF antibody
Anti-XBR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance