| Function | Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways.affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG.the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin.polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of pro-inflammatory cytokines and IFN beta in LPS-tolerized macrophages. |
| Protein Name | Tumor Necrosis Factor Alpha-Induced Protein 3Tnf Alpha-Induced Protein 3Otu Domain-Containing Protein 7cPutative Dna-Binding Protein A20Zinc Finger Protein A20 Cleaved Into - A20p50 - A20p37 |
| Database Links | Reactome: R-HSA-168638Reactome: R-HSA-5357786Reactome: R-HSA-5357905Reactome: R-HSA-5357956Reactome: R-HSA-5689896Reactome: R-HSA-936440 |
| Cellular Localisation | CytoplasmNucleusLysosomeA20p50: Cytoplasm |
| Alternative Antibody Names | Anti-Tumor Necrosis Factor Alpha-Induced Protein 3 antibodyAnti-Tnf Alpha-Induced Protein 3 antibodyAnti-Otu Domain-Containing Protein 7c antibodyAnti-Putative Dna-Binding Protein A20 antibodyAnti-Zinc Finger Protein A20 Cleaved Into - A20p50 - A20p37 antibodyAnti-TNFAIP3 antibodyAnti-OTUD7C antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and TNFAIP3 knockout (KO) A-549 cells, using [KO Validated] TNFAIP3 Rabbit pAb (STJ11100029) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89101/405568/STJ11100029_1__25038.1744891337.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TNFAIP3 knockout (KO) A-549 cells, using [KO Validated] TNFAIP3 Rabbit pAb (STJ11100029) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.")
