| Post Translational Modifications | Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms. |
| Function | Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409'. Cleaves and activates caspase-7 (CASP7). |
| Protein Name | Calpain-1 Catalytic SubunitCalcium-Activated Neutral Proteinase 1Canp 1Calpain Mu-TypeCalpain-1 Large SubunitCell Proliferation-Inducing Gene 30 ProteinMicromolar-CalpainMucanp |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-6798695Reactome: R-HSA-6809371Reactome: R-HSA-8862803 |
| Cellular Localisation | CytoplasmCell MembraneTranslocates To The Plasma Membrane Upon Ca(2+) BindingIn Granular Keratinocytes And In Lower CorneocytesColocalizes With Flg And Flg2 |
| Alternative Antibody Names | Anti-Calpain-1 Catalytic Subunit antibodyAnti-Calcium-Activated Neutral Proteinase 1 antibodyAnti-Canp 1 antibodyAnti-Calpain Mu-Type antibodyAnti-Calpain-1 Large Subunit antibodyAnti-Cell Proliferation-Inducing Gene 30 Protein antibodyAnti-Micromolar-Calpain antibodyAnti-Mucanp antibodyAnti-CAPN1 antibodyAnti-CANPL1 antibodyAnti-PIG30 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and Calpain 1 knockout (KO) HeLa cells, using [KO Validated] Calpain 1 Rabbit polyclonal antibody (STJ119979) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/98764/382816/STJ119979_1__25837.1713151311.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Calpain 1 knockout (KO) HeLa cells, using [KO Validated] Calpain 1 Rabbit polyclonal antibody (STJ119979) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")