• Western blot analysis of extracts of various cell lines, using RAG2 antibody (STJ114362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RAG2 antibody (258-527) (STJ114362)

SKU:
STJ114362

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAG2 (258-527) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAG2
Gene ID: 5897
Uniprot ID: RAG2_HUMAN
Immunogen Region: 258-527
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 258-527 of human RAG2 (NP_001230715.1).
Immunogen Sequence: VSSAILTQTNNDEFVIVGGY QLENQKRMICNIISLEDNKI EIREMETPDWTPDIKHSKIW FGSNMGNGTVFLGIPGDNKQ VVSEGFYFYMLKCAEDDTNE EQTTFTNSQTSTEDPGDSTP FEDSEEFCFSAEANSFDGDD EFDTYNEDDEEDESETGYWI TCCPTCDVDINTWVPFYSTE LNKPAMIYCSHGDGHWVHAQ CMDLAERTLIHLSAGSNKYY CNEHVEIARALHTPQRVLP
Tissue Specificity Cells of the B- and T-lymphocyte lineages.
Function Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3.
Protein Name V(Dj Recombination-Activating Protein 2
Rag-2
Database Links Reactome: R-HSA-1266695
Reactome: R-HSA-5687128
Cellular Localisation Nucleus
Alternative Antibody Names Anti-V(Dj Recombination-Activating Protein 2 antibody
Anti-Rag-2 antibody
Anti-RAG2 antibody

Information sourced from Uniprot.org

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