• Western blot analysis of extracts of various cell lines, using RACK1 Rabbit monoclonal antibody (STJ11101953) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using RACK1 Rabbit monoclonal antibody (STJ11101953) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RACK1 antibody (220-310) [S3MR] (STJ11101953)

SKU:
STJ11101953

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-RACK1 (220-310) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RACK1
Gene ID: 10399
Uniprot ID: RACK1_HUMAN
Immunogen Region: 220-310
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 220-310 of human RACK1 (P63244).
Immunogen Sequence: DLNEGKHLYTLDGGDIINAL CFSPNRYWLCAATGPSIKIW DLEGKIIVDELKQEVISTSS KAEPPQCTSLAWSADGQTLF AGYTDNLVRVW
Tissue Specificity In the liver, expressed at higher levels in activated hepatic stellate cells than in hepatocytes or Kupffer cells. Up-regulated in hepatocellular carcinomas and in the adjacent non-tumor liver tissue.
Post Translational Modifications Phosphorylated on Tyr-228 and/or Tyr-246 by SRC. This is required for binding to SRC. (Microbial infection) Phosphorylated by vaccinia virus B1 kinase on serine and threonine residues.this phosphorylation remodels the ribosome properties, favoring the viral mRNA translation.
Function Scaffolding protein involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression. Involved in the initiation of the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, by promoting ubiquitination of a subset of 40S ribosomal subunits. Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. Involved in the transport of ABCB4 from the Golgi to the apical bile canalicular membrane. Promotes migration of breast carcinoma cells by binding to and activating RHOA. Acts as an adapter for the dephosphorylation and inactivation of AKT1 by promoting recruitment of PP2A phosphatase to AKT1. (Microbial infection) Binds to Y.pseudotuberculosis yopK which leads to inhibition of phagocytosis and survival of bacteria following infection of host cells. (Microbial infection) Enhances phosphorylation of HIV-1 Nef by PKCs. (Microbial infection) In case of poxvirus infection, remodels the ribosomes so that they become optimal for the viral mRNAs (containing poly-A leaders) translation but not for host mRNAs. (Microbial infection) Contributes to the cap-independent internal ribosome entry site (IRES)-mediated translation by some RNA viruses.
Protein Name Small Ribosomal Subunit Protein Rack1
Cell Proliferation-Inducing Gene 21 Protein
Guanine Nucleotide-Binding Protein Subunit Beta-2-Like 1
Guanine Nucleotide-Binding Protein Subunit Beta-Like Protein 12.3
Human Lung Cancer Oncogene 7 Protein
Hlc-7
Receptor For Activated C Kinase
Receptor Of Activated Protein C Kinase 1 Cleaved Into - Small Ribosomal Subunit Protein Rack1 - N-Terminally Processed
Guanine Nucleotide-Binding Protein Subunit Beta-2-Like 1 - N-Terminally Processed
Receptor Of Activated Protein C Kinase 1 - N-Terminally Processed
Database Links Reactome: R-HSA-5357905
Reactome: R-HSA-5357956
Reactome: R-HSA-5626978
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasm
Perinuclear Region
Nucleus
Perikaryon
Cell Projection
Dendrite
Phagocytic Cup
Recruited To The Plasma Membrane Through Interaction With Krt1 Which Binds To Membrane-Bound Itgb1
Also Associated With The Membrane In Oncogene-Transformed Cells
Pkc Activation Induces Translocation From The Perinuclear Region To The Cell Periphery
In The Brain
Detected Mainly In Cell Bodies And Dendrites With Little Expression In Axonal Fibers Or Nuclei
Localized To Phagocytic Cups Following Infection By Y
Pestis
Alternative Antibody Names Anti-Small Ribosomal Subunit Protein Rack1 antibody
Anti-Cell Proliferation-Inducing Gene 21 Protein antibody
Anti-Guanine Nucleotide-Binding Protein Subunit Beta-2-Like 1 antibody
Anti-Guanine Nucleotide-Binding Protein Subunit Beta-Like Protein 12.3 antibody
Anti-Human Lung Cancer Oncogene 7 Protein antibody
Anti-Hlc-7 antibody
Anti-Receptor For Activated C Kinase antibody
Anti-Receptor Of Activated Protein C Kinase 1 Cleaved Into - Small Ribosomal Subunit Protein Rack1 - N-Terminally Processed antibody
Anti-Guanine Nucleotide-Binding Protein Subunit Beta-2-Like 1 - N-Terminally Processed antibody
Anti-Receptor Of Activated Protein C Kinase 1 - N-Terminally Processed antibody
Anti-RACK1 antibody
Anti-GNB2L1 antibody
Anti-HLC7 antibody
Anti-PIG21 antibody

Information sourced from Uniprot.org

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