| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Rabring 7/BCA2 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000. WB: Approx 35kDa band observed in Human lysates of cell line HEPG2 (calculated MW of 33.7kDa according to NP_055270.1). Recommended concentration: 0.3-1µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | RNF115 |
| Gene ID: | 27246 |
| Uniprot ID: | RN115_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_055270.1 |
| Immunogen Sequence: | DQDNRANERGHQTH |
| Post Translational Modifications | Phosphorylated by AKT1, allowing association with the 14-3-3 chaperones that facilitates associating with TLRs. RING-type zinc finger-dependent and E2-dependent autoubiquitination. Deubiquitinated by USP9X.antogonizing its autoubiquitination and subsequent proteasomal degradation. |
| Function | E3 ubiquitin-protein ligase that catalyzes the 'Lys-48'- and/or 'Lys-63'-linked polyubiquitination of various substrates and thereby plays a role in a number of signaling pathways including autophagy, innate immunity, cell proliferation and cell death. Plays a role in the endosomal trafficking and degradation of membrane receptors including EGFR, FLT3, MET and CXCR4 through their polyubiquitination. Participates together with BST2 in antiviral immunity by facilitating the internalization of HIV-1 virions into intracellular vesicles leading to their lysosomal degradation. Possesses also an antiviral activity independently of BST2 by promoting retroviral GAG proteins ubiquitination, redistribution to endo-lysosomal compartments and, ultimately, lysosomal degradation. Catalyzes distinct types of ubiquitination on MAVS and STING1 at different phases of viral infection to promote innate antiviral response. Mediates the 'Lys-48'-linked ubiquitination of MAVS leading to its proteasomal degradation and ubiquitinates STING1 via 'Lys-63'-linked polyubiquitination, critical for its oligomerization and the subsequent recruitment of TBK1. Plays a positive role in the autophagosome-lysosome fusion by interacting with STX17 and enhancing its stability without affecting 'Lys-48'- or 'Lys-63'-linked polyubiquitination levels, which in turn promotes autophagosome maturation. Negatively regulates TLR-induced expression of proinflammatory cytokines by catalyzing 'Lys-11'-linked ubiquitination of RAB1A and RAB13 to inhibit post-ER trafficking of TLRs to the Golgi by RAB1A and subsequently from the Golgi apparatus to the cell surface by RAB13. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf115 Ring Finger Protein 115 Ring-Type E3 Ubiquitin Transferase Rnf115 Rab7-Interacting Ring Finger Protein Rabring 7 Zinc Finger Protein 364 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | Cytoplasm Nucleus Endoplasmic Reticulum Golgi Apparatus The Gtp-Bound Form Of Rab7a Recruits Rnf115 From The Cytosol Onto Late Endosomes/Lysosomes |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf115 antibody Anti-Ring Finger Protein 115 antibody Anti-Ring-Type E3 Ubiquitin Transferase Rnf115 antibody Anti-Rab7-Interacting Ring Finger Protein antibody Anti-Rabring 7 antibody Anti-Zinc Finger Protein 364 antibody Anti-RNF115 antibody Anti-ZNF364 antibody |
Information sourced from Uniprot.org