• Immunofluorescence analysis of U2OS cells using QARS antibody (STJ29040). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human gastric cancer using QARS antibody (STJ29040) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver damage using QARS antibody (STJ29040) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using QARS antibody (STJ29040) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using QARS antibody (STJ29040) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-QARS antibody (1-250) (STJ29040)

SKU:
STJ29040

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-QARS (1-250) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: QARS1
Gene ID: 5859
Uniprot ID: SYQ_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human QARS (NP_005042.1).
Immunogen Sequence: MAALDSLSLFTSLGLSEQKA RETLKNSALSAQLREAATQA QQTLGSTIDKATGILLYGLA SRLRDTRRLSFLVSYIASKK IHTEPQLSAALEYVRSHPLD PIDTVDFERECGVGVIVTPE QIEEAVEAAINRHRPQLLVE RYHFNMGLLMGEARAVLKWA DGKMIKNEVDMQVLHLLGPK LEADLEKKFKVAKARLEETD RRTAKDVVENGETADQTLSL MEQLRGEALKFHKPGENYK
Tissue Specificity Highly expressed in fetal cerebral cortex, particularly in the ventricular zone, inner subventricular zone, outer subventricular zone, and cortical plate.
Function Glutamine--tRNA ligase. Plays a critical role in brain development.
Protein Name Glutamine--Trna Ligase
Glutaminyl-Trna Synthetase
Glnrs
Database Links Reactome: R-HSA-2408522
Reactome: R-HSA-379716
Reactome: R-HSA-379726
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Glutamine--Trna Ligase antibody
Anti-Glutaminyl-Trna Synthetase antibody
Anti-Glnrs antibody
Anti-QARS1 antibody
Anti-QARS antibody

Information sourced from Uniprot.org

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