• Western blot analysis of extracts of various cell lines, using PYGM antibody (STJ111685) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using PYGM antibody (STJ111685) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PYGM antibody (743-842) (STJ111685)

SKU:
STJ111685

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PYGM
Gene ID: 5837
Uniprot ID: PYGM_HUMAN
Immunogen Region: 743-842
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 743-842 of human PYGM (NP_005600.1).
Immunogen Sequence: IEQLSSGFFSPKQPDLFKDI VNMLMHHDRFKVFADYEDYI KCQEKVSALYKNPREWTRMV IRNIATSGKFSSDRTIAQYA REIWGVEPSRQRLPAPDEAI
Post Translational Modifications Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.
Function Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.
Protein Name Glycogen Phosphorylase - Muscle Form
Myophosphorylase
Database Links Reactome: R-HSA-70221
Alternative Antibody Names Anti-Glycogen Phosphorylase - Muscle Form antibody
Anti-Myophosphorylase antibody
Anti-PYGM antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance