| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PYGB (544-843) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PYGB |
| Gene ID: |
5834 |
| Uniprot ID: |
PYGB_HUMAN |
| Immunogen Region: |
544-843 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 544-843 of human PYGB (NP_002853.2). |
| Immunogen Sequence: |
LKFSAFLEKEYKVKINPSSM FDVHVKRIHEYKRQLLNCLH VVTLYNRIKRDPAKAFVPRT VMIGGKAAPGYHMAKLIIKL VTSIGDVVNHDPVVGDRLKV IFLENYRVSLAEKVIPAADL SQQISTAGTEASGTGNMKFM LNGALTIGTMDGANVEMAEE AGAENLFIFGLRVEDVEALD RKGYNAREYYDHLPELKQAV DQISSGFFSPKEPDCFKDIV NMLMHHDRFKVFADYEAYM |
| Post Translational Modifications | Phosphorylated. Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A. |
| Function | Glycogen phosphorylase that regulates glycogen mobilization. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. |
| Protein Name | Glycogen Phosphorylase - Brain Form |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-70221 |
| Alternative Antibody Names | Anti-Glycogen Phosphorylase - Brain Form antibodyAnti-PYGB antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and PYGB knockout (KO) HeLa cells, using [KO Validated] PYGB Rabbit polyclonal antibody (STJ11100786) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89722/358203/STJ11100786_1__04144.1713122524.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PYGB knockout (KO) HeLa cells, using [KO Validated] PYGB Rabbit polyclonal antibody (STJ11100786) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
