• Immunofluorescence analysis of MCF-7 cells using PTRH2 antibody (STJ28549). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using PTRH2 antibody (STJ28549) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using PTRH2 antibody (STJ28549) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PTRH2 antibody (STJ28549) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PTRH2 antibody (40-179) (STJ28549)

SKU:
STJ28549

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:10-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTRH2
Gene ID: 51651
Uniprot ID: PTH2_HUMAN
Immunogen Region: 40-179
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 40-179 of human PTRH2 (NP_057161.1).
Immunogen Sequence: LPKSKTSKTHTDTESEASIL GDSGEYKMILVVRNDLKMGK GKVAAQCSHAAVSAYKQIQR RNPEMLKQWEYCGQPKVVVK APDEETLIALLAHAKMLGLT VSLIQDAGRTQIAPGSQTVL GIGPGPADLIDKVTGHLKLY
Post Translational Modifications Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.
Function Peptidyl-tRNA hydrolase which releases tRNAs from the ribosome during protein synthesis. Promotes caspase-independent apoptosis by regulating the function of two transcriptional regulators, AES and TLE1.
Protein Name Peptidyl-Trna Hydrolase 2 - Mitochondrial
Pth 2
Bcl-2 Inhibitor Of Transcription 1
Database Links Reactome: R-HSA-5689880
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Peptidyl-Trna Hydrolase 2 - Mitochondrial antibody
Anti-Pth 2 antibody
Anti-Bcl-2 Inhibitor Of Transcription 1 antibody
Anti-PTRH2 antibody
Anti-BIT1 antibody
Anti-PTH2 antibody
Anti-CGI-147 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance