• Western blot analysis of extracts of various cell lines, using PRSS1 antibody (STJ25163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
  • Immunohistochemistry of paraffin-embedded human lung cancer using PRSS1 antibody (STJ25163) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human prostate using PRSS1 antibody (STJ25163) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse lung using PRSS1 antibody (STJ25163) at dilution of 1:100 (40x lens).

Anti-PRSS1 antibody (24-247) (STJ25163)

SKU:
STJ25163

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:100-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRSS1
Gene ID: 5644
Uniprot ID: TRY1_HUMAN
Immunogen Region: 24-247
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 24-247 of human PRSS1 (NP_002760.1).
Immunogen Sequence: IVGGYNCEENSVPYQVSLNS GYHFCGGSLINEQWVVSAGH CYKSRIQVRLGEHNIEVLEG NEQFINAAKIIRHPQYDRKT LNNDIMLIKLSSRAVINARV STISLPTAPPATGTKCLISG WGNTASSGADYPDELQCLDA PVLSQAKCEASYPGKITSNM FCVGFLEGGKDSCQGDSGGP VVCNGQLQGVVSWGDGCAQK NKPGVYTKVYNYVKWIKNTI AANS
Post Translational Modifications Occurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122. Sulfation at Tyr-154 increases selectivity towards basic versus apolar residues at the P2' position of inhibitors that bind in a substrate-like fashion. Although the increase in selectivity is relatively small, it may facilitate digestion of a broader range of dietary proteins.
Function Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.
Protein Name Serine Protease 1
Anionic Trypsin I
Anionic Trypsin-I
Beta-Trypsin
Cationic Trypsinogen
Pretrypsinogen I
Trypsin I
Trypsin-1 Cleaved Into - Alpha-Trypsin Chain 1 - Alpha-Trypsin Chain 2
Database Links Reactome: R-HSA-1592389
Reactome: R-HSA-9758881
Cellular Localisation Secreted
Extracellular Space
Alternative Antibody Names Anti-Serine Protease 1 antibody
Anti-Anionic Trypsin I antibody
Anti-Anionic Trypsin-I antibody
Anti-Beta-Trypsin antibody
Anti-Cationic Trypsinogen antibody
Anti-Pretrypsinogen I antibody
Anti-Trypsin I antibody
Anti-Trypsin-1 Cleaved Into - Alpha-Trypsin Chain 1 - Alpha-Trypsin Chain 2 antibody
Anti-PRSS1 antibody
Anti-TRP1 antibody
Anti-TRY1 antibody
Anti-TRYP1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance