• Immunohistochemistry analysis of CD86 in paraffin-embedded mouse spleen using CD86 Rabbit polyclonal antibody (STJ113561) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CD86 Rabbit polyclonal antibody (STJ113561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 300s.

Anti-CD86 antibody (24-247) (STJ113561)

SKU:
STJ113561

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD86 (24-247) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD86
Gene ID: 942
Uniprot ID: CD86_HUMAN
Immunogen Region: 24-247
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-247 of human CD86 (NP_787058.4).
Immunogen Sequence: APLKIQAYFNETADLPCQFA NSQNQSLSELVVFWQDQENL VLNEVYLGKEKFDSVHSKYM GRTSFDSDSWTLRLHNLQIK DKGLYQCIIHHKKPTGMIRI HQMNSELSVLANFSQPEIVP ISNITENVYINLTCSSIHGY PEPKKMSVLLRTKNSTIEYD GIMQKSQDNVTELYDVSISL SVSFPDVTSNMTIFCILETD KTRLLSSPFSIELEDPQPPP DHIP
Tissue Specificity Expressed by activated B-lymphocytes and monocytes.
Post Translational Modifications Polyubiquitinated.which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
Function Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation. Isoform 2: Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
Protein Name T-Lymphocyte Activation Antigen Cd86
Activation B7-2 Antigen
B70
Bu63
Ctla-4 Counter-Receptor B7.2
Fun-1
Cd Antigen Cd86
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-2219530
Reactome: R-HSA-389356
Reactome: R-HSA-389357
Reactome: R-HSA-389359
Reactome: R-HSA-389513
Reactome: R-HSA-6783783
Reactome: R-HSA-6811558
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-T-Lymphocyte Activation Antigen Cd86 antibody
Anti-Activation B7-2 Antigen antibody
Anti-B70 antibody
Anti-Bu63 antibody
Anti-Ctla-4 Counter-Receptor B7.2 antibody
Anti-Fun-1 antibody
Anti-Cd Antigen Cd86 antibody
Anti-CD86 antibody
Anti-CD28LG2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance