• Western blot analysis of lysates from wild type (WT) and PRMT8 knockout (KO) 293T cells, using [KO Validated] PRMT8 Rabbit polyclonal antibody (STJ11100394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PRMT8 antibody (200-380) (STJ11100394)

SKU:
STJ11100394

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRMT8 (200-380) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRMT8
Gene ID: 56341
Uniprot ID: ANM8_HUMAN
Immunogen Region: 200-380
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-380 of human PRMT8 (NP_062828.3).
Immunogen Sequence: VIFARDKWLKPGGLMFPDRA ALYVVAIEDRQYKDFKIHWW ENVYGFDMTCIRDVAMKEPL VDIVDPKQVVTNACLIKEVD IYTVKTEELSFTSAFCLQIQ RNDYVHALVTYFNIEFTKCH KKMGFSTAPDAPYTHWKQTV FYLEDYLTVRRGEEIYGTIS MKPNAKNVRDLDFTVDLDFK G
Tissue Specificity Brain-specific.
Function S-adenosyl-L-methionine-dependent and membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA) in proteins such as NIFK, myelin basic protein, histone H4, H2A and H2A/H2B dimer. Able to mono- and dimethylate EWS protein.however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS.
Protein Name Protein Arginine N-Methyltransferase 8
Heterogeneous Nuclear Ribonucleoprotein Methyltransferase-Like Protein 4
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Alternative Antibody Names Anti-Protein Arginine N-Methyltransferase 8 antibody
Anti-Heterogeneous Nuclear Ribonucleoprotein Methyltransferase-Like Protein 4 antibody
Anti-PRMT8 antibody
Anti-HRMT1L3 antibody
Anti-HRMT1L4 antibody

Information sourced from Uniprot.org

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