| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PRIM2 (1-170) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PRIM2 |
| Gene ID: |
5558 |
| Uniprot ID: |
PRI2_HUMAN |
| Immunogen Region: |
1-170 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human PRIM2 (NP_000938.2). |
| Immunogen Sequence: |
MEFSGRKWRKLRLAGDQRNA SYPHCLQFYLQPPSENISLI EFENLAIDRVKLLKSVENLG VSYVKGTEQYQSKLESELRK LKFSYRENLEDEYEPRRRDH ISHFILRLAYCQSEELRRWF IQQEMDLLRFRFSILPKDKI QDFLKDSQLQFEAISDEEKT LREQEIVASS |
| Function | Regulatory subunit of the DNA primase complex and component of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which play an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. In the primase complex, both subunits are necessary for the initial di-nucleotide formation, but the extension of the primer depends only on the catalytic subunit. Binds RNA:DNA duplex and coordinates the catalytic activities of PRIM1 and POLA2 during primase-to-polymerase switch. |
| Protein Name | Dna Primase Large SubunitDna Primase 58 Kda SubunitP58 |
| Database Links | Reactome: R-HSA-113501Reactome: R-HSA-174411Reactome: R-HSA-174430Reactome: R-HSA-68952Reactome: R-HSA-68962Reactome: R-HSA-69091Reactome: R-HSA-69166Reactome: R-HSA-69183Reactome: R-HSA-9710421 |
| Alternative Antibody Names | Anti-Dna Primase Large Subunit antibodyAnti-Dna Primase 58 Kda Subunit antibodyAnti-P58 antibodyAnti-PRIM2 antibodyAnti-PRIM2A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
