| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PRIM1 (121-420) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PRIM1 |
| Gene ID: |
5557 |
| Uniprot ID: |
PRI1_HUMAN |
| Immunogen Region: |
121-420 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 121-420 of human PRIM1 (NP_000937.1). |
| Immunogen Sequence: |
CCSSADICPKCWTLMTMAIR IIDRALKEDFGFKHRLWVYS GRRGVHCWVCDESVRKLSSA VRSGIVEYLSLVKGGQDVKK KVHLSEKIHPFIRKSINIIK KYFEEYALVNQDILENKESW DKILALVPETIHDELQQSFQ KSHNSLQRWEHLKKVASRYQ NNIKNDKYGPWLEWEIMLQY CFPRLDINVSKGINHLLKSP FSVHPKTGRISVPIDLQKVD QFDPFTVPTISFICRELDA |
| Function | Catalytic subunit of the DNA primase complex and component of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex - primosome/replisome) which play an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. In the primase complex, both subunits are necessary for the initial di-nucleotide formation, but the extension of the primer depends only on the catalytic subunit. Synthesizes 9-mer RNA primers (also known as the 'unit length' RNA primers). Incorporates only ribonucleotides in the presence of ribo- and deoxy-nucleotide triphosphates (rNTPs, dNTPs). Requires template thymine or cytidine to start the RNA primer synthesis, with an adenine or guanine at its 5'-end. Binds single stranded DNA. |
| Protein Name | Dna Primase Small SubunitDna Primase 49 Kda SubunitP49 |
| Database Links | Reactome: R-HSA-113501Reactome: R-HSA-174411Reactome: R-HSA-174430Reactome: R-HSA-68952Reactome: R-HSA-68962Reactome: R-HSA-69091Reactome: R-HSA-69166Reactome: R-HSA-69183Reactome: R-HSA-9710421 |
| Alternative Antibody Names | Anti-Dna Primase Small Subunit antibodyAnti-Dna Primase 49 Kda Subunit antibodyAnti-P49 antibodyAnti-PRIM1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
