• Western blot analysis of extracts of rat heart, using PRDM2 antibody (STJ111300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using PRDM2 antibody (STJ111300) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat testis using PRDM2 antibody (STJ111300) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of rat heart, using PRDM2 antibody (STJ111300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PRDM2 antibody (1-240) (STJ111300)

SKU:
STJ111300

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Host: Rabbit
Applications: WB/IHC
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRDM2 (1-240) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRDM2
Gene ID: 7799
Uniprot ID: PRDM2_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human PRDM2 (NP_036363.2).
Immunogen Sequence: MNQNTTEPVAATETLAEVPE HVLRGLPEEVRLFPSAVDKT RIGVWATKPILKGKKFGPFV GDKKKRSQVKNNVYMWEVYY PNLGWMCIDATDPEKGNWLR YVNWACSGEEQNLFPLEINR AIYYKTLKPIAPGEELLVWY NGEDNPEIAAAIEEERASAR SKRSSPKSRKGKKKSQENKN KGNKIQDIQLKTSEPDFTSA NMRDSAEGPKEDEEKPSASA LEQPATLQEVASQEVPPEL
Tissue Specificity Highly expressed in retinoblastoma cell lines and in brain tumors. Also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. Isoform 1 is expressed in testis at much higher level than isoform 3.
Function S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene.
Protein Name Pr Domain Zinc Finger Protein 2
Gata-3-Binding Protein G3b
Lysine N-Methyltransferase 8
Mtb-Zf
Mte-Binding Protein
Pr Domain-Containing Protein 2
Retinoblastoma Protein-Interacting Zinc Finger Protein
Zinc Finger Protein Riz
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pr Domain Zinc Finger Protein 2 antibody
Anti-Gata-3-Binding Protein G3b antibody
Anti-Lysine N-Methyltransferase 8 antibody
Anti-Mtb-Zf antibody
Anti-Mte-Binding Protein antibody
Anti-Pr Domain-Containing Protein 2 antibody
Anti-Retinoblastoma Protein-Interacting Zinc Finger Protein antibody
Anti-Zinc Finger Protein Riz antibody
Anti-PRDM2 antibody
Anti-KMT8 antibody
Anti-RIZ antibody

Information sourced from Uniprot.org

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