| Host: | Rabbit |
| Applications: | WB/IHC-F/IHC-P/ICC/IF/IP |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit monoclonal antibody anti-PP1C beta (314-327 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality : | Monoclonal |
| Clone ID : | R09-6B7 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 50mM Tris-Glycine (pH7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Purification: | Affinity Purified |
| Concentration: | 0.3 mg/ml |
| Dilution Range: | WB 1:500-1:1000 IHC 1:50-1:100 IF 1:50-1:200 IP 1:20 |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | PPP1CB |
| Gene ID: | 5500 |
| Uniprot ID: | PP1B_HUMAN |
| Immunogen Region: | 314-327 aa |
| Immunogen: | A synthetic peptide of human PPP1CB |
| Function | Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation. The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities. The SMP complex enhances the dephosphorylation activity and substrate specificity of PP1c. |
| Protein Name | Serine/Threonine-Protein Phosphatase Pp1-Beta Catalytic Subunit Pp-1b Ppp1cd |
| Database Links | Reactome: R-HSA-163560 Reactome: R-HSA-2173788 Reactome: R-HSA-2565942 Reactome: R-HSA-400253 Reactome: R-HSA-5625740 Reactome: R-HSA-5625900 Reactome: R-HSA-5627117 Reactome: R-HSA-5627123 Reactome: R-HSA-5673000 Reactome: R-HSA-9726840 Reactome: R-HSA-9726842 Reactome: R-HSA-9828806 |
| Cellular Localisation | Cytoplasm Nucleus Nucleoplasm Nucleolus Highly Mobile In Cells And Can Be Relocalized Through Interaction With Targeting Subunits In The Presence Of Ppp1r8 Relocalizes From The Nucleus To Nuclear Speckles |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Phosphatase Pp1-Beta Catalytic Subunit antibody Anti-Pp-1b antibody Anti-Ppp1cd antibody Anti-PPP1CB antibody |
Information sourced from Uniprot.org