• Immunofluorescence analysis of A-431 cells using [KD Validated] PGC1 Alpha Rabbit polyclonal antibody (STJ11104718) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using [KD Validated] PGC1 Alpha Rabbit polyclonal antibody (STJ11104718) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PGC1 Alpha in paraffin-embedded rat kidney using [KO Validated] PGC1 Alpha Rabbit polyclonal antibody (STJ11104718) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PGC1 Alpha in paraffin-embedded mouse kidney using [KO Validated] PGC1 Alpha Rabbit polyclonal antibody (STJ11104718) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PGC1 Alpha in paraffin-embedded human colon using [KO Validated] PGC1 Alpha Rabbit polyclonal antibody (STJ11104718) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and PGC1 Alpha Rabbit polyclonal antibody knockdown (KD) HeLa cells, using [KD Validated] PGC1 Alpha Rabbit polyclonal antibody (STJ11104718) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates, using [KD Validated] PGC1 Alpha Rabbit polyclonal antibody (STJ11104718) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PPARGC1A antibody (611-710) (STJ11104718)

SKU:
STJ11104718

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-Alpha (611-710) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPARGC1A
Gene ID: 10891
Uniprot ID: PRGC1_HUMAN
Immunogen Region: 611-710
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 611-710 of human PGC1alpha (NP_037393.1).
Immunogen Sequence: RTHRNSPLYVRSRSRSPYSR RPRYDSYEEYQHERLKREEY RREYEKRESERAKQRERQRQ KAIEERRVIYVGKIRPDTTR TELRDRFEVFGEIEECTVNL
Tissue Specificity Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects.
Post Translational Modifications Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2. Heavily acetylated by KAT2A/GCN5 under conditions of high nutrients, leading to inactivation of PPARGC1A. Deacetylated by SIRT1 in low nutrients/high NAD conditions, leading to its activation. Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation.
Function Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Acts as a key regulator of gluconeogenesis: stimulates hepatic gluconeogenesis by increasing the expression of gluconeogenic enzymes, and acting together with FOXO1 to promote the fasting gluconeogenic program. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.
Protein Name Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-Alpha
Pgc-1-Alpha
Ppar-Gamma Coactivator 1-Alpha
Ppargc-1-Alpha
Ligand Effect Modulator 6
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-2151201
Reactome: R-HSA-2151209
Reactome: R-HSA-381340
Reactome: R-HSA-3899300
Reactome: R-HSA-400253
Reactome: R-HSA-8939902
Reactome: R-HSA-9615017
Reactome: R-HSA-9707616
Cellular Localisation Isoform 1: Nucleus
Nucleus
Pml Body
Isoform B4: Nucleus
Isoform B4-8a: Cytoplasm
Isoform B5: Nucleus
Isoform 9: Nucleus
Alternative Antibody Names Anti-Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-Alpha antibody
Anti-Pgc-1-Alpha antibody
Anti-Ppar-Gamma Coactivator 1-Alpha antibody
Anti-Ppargc-1-Alpha antibody
Anti-Ligand Effect Modulator 6 antibody
Anti-PPARGC1A antibody
Anti-LEM6 antibody
Anti-PGC1 antibody
Anti-PGC1A antibody
Anti-PPARGC1 antibody

Information sourced from Uniprot.org

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