• Western blot analysis of lysates from A549 cells, using PP2A-alpha Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using PP2A-alpha Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of HepG2 cells, using PP2A-alpha Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
  • Western blot analysis of A549 cells using PP2A-C Alpha Polyclonal Antibody diluted at 1:2000
  • Western blot analysis of various cells using PP2A-C Alpha Polyclonal Antibody diluted at 1:2000

Anti-PP2A-Alpha antibody (STJ95195)

SKU:
STJ95195

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, IF, ELISA
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-PP2A-Alpha antibody is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Specificity: PP2A-C alpha polyclonal antibody detects endogenous levels of PP2A-C alpha protein.
Uniprot ID: PP2AA_HUMAN
Gene ID: 5515
Gene Symbol: PPP2CA
Immunogen: Synthesized peptide derived from human PP2A-Calpha around the non-phosphorylation site of Y307.
Immunogen Region: 250-330 aa
Function PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. Mediates dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint.
Protein Name Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Alpha Isoform
Pp2a-Alpha
Replication Protein C
Rp-C
Database Links Reactome: R-HSA-113501
Reactome: R-HSA-1295596
Reactome: R-HSA-141444
Reactome: R-HSA-163685
Reactome: R-HSA-163767
Reactome: R-HSA-180024
Reactome: R-HSA-195253
Reactome: R-HSA-196299
Reactome: R-HSA-198753
Reactome: R-HSA-202670
Reactome: R-HSA-2465910
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-2995383
Reactome: R-HSA-389513
Reactome: R-HSA-432142
Reactome: R-HSA-4641262
Reactome: R-HSA-5339716
Reactome: R-HSA-5358747
Reactome: R-HSA-5358749
Reactome: R-HSA-5358751
Reactome: R-HSA-5358752
Reactome: R-HSA-5467337
Reactome: R-HSA-5467340
Reactome: R-HSA-5467348
Reactome: R-HSA-5663220
Reactome: R-HSA-5673000
Reactome: R-HSA-5675221
Reactome: R-HSA-6804757
Reactome: R-HSA-6811558
Reactome: R-HSA-68877
Reactome: R-HSA-69231
Reactome: R-HSA-69273
Reactome: R-HSA-9634600
Reactome: R-HSA-9648025
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Nucleus
Chromosome
Centromere
Cytoskeleton
Spindle Pole
In Prometaphase Cells
But Not In Anaphase Cells
Localizes At Centromeres
During Mitosis
Also Found At Spindle Poles
Centromeric Localization Requires The Presence Of Sgo2
Alternative Antibody Names Anti-Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Alpha Isoform antibody
Anti-Pp2a-Alpha antibody
Anti-Replication Protein C antibody
Anti-Rp-C antibody
Anti-PPP2CA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance