• Confocal imaging of paraffin-embedded Human liver tissue using PON1 Rabbit monoclonal antibody (STJ11101870, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Mouse liver tissue using PON1 Rabbit monoclonal antibody (STJ11101870, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Rat liver tissue using PON1 Rabbit monoclonal antibody (STJ11101870, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of PON1 in paraffin-embedded human liver using PON1 Rabbit monoclonal antibody (STJ11101870) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HepG2 cells, using PON1 Rabbit monoclonal antibody (STJ11101870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of lysates from Mouse liver, using PON1 Rabbit monoclonal antibody (STJ11101870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PON1 antibody (50-150) [S0MR] (STJ11101870)

SKU:
STJ11101870

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PON1 (50-150) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PON1
Gene ID: 5444
Uniprot ID: PON1_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PON1 (P27169).
Immunogen Sequence: TGSEDLEILPNGLAFISSGL KYPGIKSFNPNSPGKILLMD LNEEDPTVLELGITGSKFDV SSFNPHGISTFTDEDNAMYL LVVNHPDAKSTVELFKFQEE E
Tissue Specificity Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas.
Post Translational Modifications Glycosylated. The signal sequence is not cleaved. Present in two forms, form B contains a disulfide bond, form A does not.
Function Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
Protein Name Serum Paraoxonase/Arylesterase 1
Pon 1
Aromatic Esterase 1
A-Esterase 1
K-45
Serum Aryldialkylphosphatase 1
Database Links Reactome: R-HSA-2142688
Reactome: R-HSA-9754706
Cellular Localisation Secreted
Extracellular Space
Alternative Antibody Names Anti-Serum Paraoxonase/Arylesterase 1 antibody
Anti-Pon 1 antibody
Anti-Aromatic Esterase 1 antibody
Anti-A-Esterase 1 antibody
Anti-K-45 antibody
Anti-Serum Aryldialkylphosphatase 1 antibody
Anti-PON1 antibody
Anti-PON antibody

Information sourced from Uniprot.org

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