• Immunofluorescence analysis of A549 cells using POLR2J antibody (STJ115586).
  • Western blot analysis of extracts of mouse skeletal muscle, using POLR2J antibody (STJ115586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-POLR2J antibody (1-117) (STJ115586)

SKU:
STJ115586

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POLR2J (1-117) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POLR2J
Gene ID: 5439
Uniprot ID: RPB11_HUMAN
Immunogen Region: 1-117
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human POLR2J (NP_006225.1).
Immunogen Sequence: MNAPPAFESFLLFEGEKKIT INKDTKVPNACLFTINKEDH TLGNIIKSQLLKDPQVLFAG YKVPHPLEHKIIIRVQTTPD YSPQEAFTNAITDLISELSL LEERFRVAIKDKQEGIE
Tissue Specificity Ubiquitously expressed. High expression was found in heart and skeletal muscle.
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft.
Protein Name Dna-Directed Rna Polymerase Ii Subunit Rpb11-A
Rna Polymerase Ii Subunit B11-A
Rpb11a
Dna-Directed Rna Polymerase Ii Subunit J-1
Rna Polymerase Ii 13.3 Kda Subunit
Database Links Reactome: R-HSA-112382
Reactome: R-HSA-113418
Reactome: R-HSA-167152
Reactome: R-HSA-167158
Reactome: R-HSA-167160
Reactome: R-HSA-167161
Reactome: R-HSA-167162
Reactome: R-HSA-167172
Reactome: R-HSA-167200
Reactome: R-HSA-167238
Reactome: R-HSA-167242
Reactome: R-HSA-167243
Reactome: R-HSA-167246
Reactome: R-HSA-167287
Reactome: R-HSA-167290
Reactome: R-HSA-168325
Reactome: R-HSA-203927
Reactome: R-HSA-5578749
Reactome: R-HSA-5601884
Reactome: R-HSA-5617472
Reactome: R-HSA-674695
Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6796648
Reactome: R-HSA-6803529
Reactome: R-HSA-6807505
Reactome: R-HSA-72086
Reactome: R-HSA-72163
Reactome: R-HSA-72165
Reactome: R-HSA-72203
Reactome: R-HSA-73776
Reactome: R-HSA-73779
Reactome: R-HSA-75953
Reactome: R-HSA-75955
Reactome: R-HSA-76042
Reactome: R-HSA-77075
Reactome: R-HSA-8851708
Reactome: R-HSA-9018519
Reactome: R-HSA-9670095
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna-Directed Rna Polymerase Ii Subunit Rpb11-A antibody
Anti-Rna Polymerase Ii Subunit B11-A antibody
Anti-Rpb11a antibody
Anti-Dna-Directed Rna Polymerase Ii Subunit J-1 antibody
Anti-Rna Polymerase Ii 13.3 Kda Subunit antibody
Anti-POLR2J antibody
Anti-POLR2J1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance