| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-POFUT1 (28-194) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
POFUT1 |
| Gene ID: |
23509 |
| Uniprot ID: |
OFUT1_HUMAN |
| Immunogen Region: |
28-194 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 28-194 of human POFUT1 (NP_758436.1). |
| Immunogen Sequence: |
SWDPAGYLLYCPCMGRFGNQ ADHFLGSLAFAKLLNRTLAV PPWIEYQHHKPPFTNLHVSY QKYFKLEPLQAYHRVISLED FMEKLAPTHWPPEKRVAYCF EVAAQRSPDKKTCPMKEGNP FGPFWDQFHVSFNKSELFTG ISFSASYREQWSQRRENHSC VTLLFPR |
| Tissue Specificity | Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
| Post Translational Modifications | N-glycosylated. |
| Function | Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-S/T-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs). |
| Protein Name | Gdp-Fucose Protein O-Fucosyltransferase 1Peptide-O-Fucosyltransferase 1O-Fuct-1 |
| Database Links | Reactome: R-HSA-1912399 |
| Cellular Localisation | Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-Gdp-Fucose Protein O-Fucosyltransferase 1 antibodyAnti-Peptide-O-Fucosyltransferase 1 antibodyAnti-O-Fuct-1 antibodyAnti-POFUT1 antibodyAnti-FUT12 antibodyAnti-KIAA0180 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
