• Western blot analysis of extracts of various cell lines, using PLD1 antibody (STJ117275) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PLD1 antibody (875-1036) (STJ117275)

SKU:
STJ117275

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PLD1 (875-1036) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLD1
Gene ID: 5337
Uniprot ID: PLD1_HUMAN
Immunogen Region: 875-1036
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 875-1036 of human PLD1 (NP_001123553.1).
Immunogen Sequence: NINDRSMLGKRDSEMAVIVQ DTETVPSVMDGKEYQAGRFA RGLRLQCFRVVLGYLDDPSE DIQDPVSDKFFKEVWVSTAA RNATIYDKVFRCLPNDEVHN LIQLRDFINKPVLAKEDPIR AEEELKKIRGFLVQFPFYFL SEESLLPSVGTKEAIVPMEV WT
Tissue Specificity Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
Function Function as phospholipase selective for phosphatidylcholine. Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.
Protein Name Phospholipase D1
Pld 1
Hpld1
Choline Phosphatase 1
Phosphatidylcholine-Hydrolyzing Phospholipase D1
Database Links Reactome: R-HSA-1483148
Reactome: R-HSA-1483166
Reactome: R-HSA-2029485
Reactome: R-HSA-6798695
Reactome: R-HSA-8980692
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013408
Cellular Localisation Cytoplasm
Perinuclear Region
Endoplasmic Reticulum Membrane
Lipid-Anchor
Cytoplasmic Side
Golgi Apparatus Membrane
Late Endosome Membrane
Alternative Antibody Names Anti-Phospholipase D1 antibody
Anti-Pld 1 antibody
Anti-Hpld1 antibody
Anti-Choline Phosphatase 1 antibody
Anti-Phosphatidylcholine-Hydrolyzing Phospholipase D1 antibody
Anti-PLD1 antibody

Information sourced from Uniprot.org

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