• Western blot analysis of extracts of various cell lines, using OGT antibody (STJ111128) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-OGT antibody (857-1036) (STJ111128)

SKU:
STJ111128

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-OGT (857-1036) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OGT
Gene ID: 8473
Uniprot ID: OGT1_HUMAN
Immunogen Region: 857-1036
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 857-1036 of human OGT (NP_858059.1).
Immunogen Sequence: RVPNSVLWLLRFPAVGEPNI QQYAQNMGLPQNRIIFSPVA PKEEHVRRGQLADVCLDTPL CNGHTTGMDVLWAGTPMVTM PGETLASRVAASQLTCLGCL ELIAKNRQEYEDIAVKLGTD LEYLKKVRGKVWKQRISSPL FNTKQYTMELERLYLQMWEH YAAGNKPDHMIKPVEVTESA
Tissue Specificity Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
Post Translational Modifications Ubiquitinated, leading to its proteasomal degradation. Phosphorylation on Ser-3 or Ser-4 by GSK3-beta positively regulates its activity.
Function Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, ATG4B, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU, NOD2 and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Probably by glycosylating KMT2E/MLL5, stabilizes KMT2E/MLL5 by preventing its ubiquitination. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O-GlcNAcylation of 'Ser-112' of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3). As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues. O-GlcNAcylation of 'Ser-75' of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex. Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver. Stabilizes clock proteins BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation. Promotes the CLOCK-BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2. O-glycosylates HCFC1 and regulates its proteolytic processing and transcriptional activity. Regulates mitochondrial motility in neurons by mediating glycosylation of TRAK1. Glycosylates HOXA1. O-glycosylates FNIP1. Promotes autophagy by mediating O-glycosylation of ATG4B. Isoform 2: The mitochondrial isoform (mOGT) is cytotoxic and triggers apoptosis in several cell types including INS1, an insulinoma cell line.
Protein Name Udp-N-Acetylglucosamine--Peptide N-Acetylglucosaminyltransferase 110 Kda Subunit
O-Glcnac Transferase Subunit P110
O-Linked N-Acetylglucosamine Transferase 110 Kda Subunit
Ogt
Database Links Reactome: R-HSA-3214847
Reactome: R-HSA-5213460
Reactome: R-HSA-5675482
Reactome: R-HSA-5689603
Reactome: R-HSA-9772755
Cellular Localisation Nucleus
Cytoplasm
Predominantly Localizes To The Nucleus
Isoform 2: Mitochondrion
Membrane
Associates With The Mitochondrial Inner Membrane
Isoform 3: Cytoplasm
Cell Membrane
Mitochondrion Membrane
Cell Projection
Mostly In The Nucleus
Retained In The Nucleus Via Interaction With Hcfc1
After Insulin Induction
Translocated From The Nucleus To The Cell Membrane Via Phosphatidylinositide Binding
Colocalizes With Akt1 At The Plasma Membrane
Trak1 Recruits This Protein To Mitochondria
In The Absence Of Trak1
Localizes In Cytosol And Nucleus
Isoform 4: Cytoplasm
Alternative Antibody Names Anti-Udp-N-Acetylglucosamine--Peptide N-Acetylglucosaminyltransferase 110 Kda Subunit antibody
Anti-O-Glcnac Transferase Subunit P110 antibody
Anti-O-Linked N-Acetylglucosamine Transferase 110 Kda Subunit antibody
Anti-Ogt antibody
Anti-OGT antibody

Information sourced from Uniprot.org

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