• Immunohistochemistry analysis of paraffin-embedded mouse lung using PI3 antibody (STJ27394) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using PI3 antibody (STJ27394) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PI3 antibody (STJ27394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-PI3 antibody (23-117) (STJ27394)

SKU:
STJ27394

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PI3 (23-117) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PI3
Gene ID: 5266
Uniprot ID: ELAF_HUMAN
Immunogen Region: 23-117
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-117 of human PI3 (NP_002629.1).
Immunogen Sequence: AVTGVPVKGQDTVKGRVPFN GQDPVKGQVSVKGQDKVKAQ EPVKGPVSTKPGSCPIILIR CAMLNPPNRCLKDTDCPGIK KCCEGSCGMACFVPQ
Function Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. Has been shown to inhibit the alpha-4-beta-2/CHRNA2-CHRNB2 nicotinic acetylcholine receptor and to produce a weak inhibition on Kv11.1/KCNH2/ERG1 and on the transient receptor potential cation channel subfamily V member 1 (TRPV1).
Protein Name Elafin
Elastase-Specific Inhibitor
Esi
Peptidase Inhibitor 3
Pi-3
Protease Inhibitor Wap3
Skin-Derived Antileukoproteinase
Skalp
Wap Four-Disulfide Core Domain Protein 14
Database Links Reactome: R-HSA-6803157
Reactome: R-HSA-6809371
Cellular Localisation Secreted
Alternative Antibody Names Anti-Elafin antibody
Anti-Elastase-Specific Inhibitor antibody
Anti-Esi antibody
Anti-Peptidase Inhibitor 3 antibody
Anti-Pi-3 antibody
Anti-Protease Inhibitor Wap3 antibody
Anti-Skin-Derived Antileukoproteinase antibody
Anti-Skalp antibody
Anti-Wap Four-Disulfide Core Domain Protein 14 antibody
Anti-PI3 antibody
Anti-WAP3 antibody
Anti-WFDC14 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance