• Western blot analysis of extracts of various cell lines, using PHOSPHO2 antibody (STJ114682) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunofluorescence analysis of NIH/3T3 cells using PHOSPHO2 rabbit polyclonal antibody (STJ114682) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using PHOSPHO2 rabbit polyclonal antibody (STJ114682) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PHOSPHO2 rabbit polyclonal antibody (STJ114682) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PHOSPHO2 antibody (1-100) (STJ114682)

SKU:
STJ114682

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PHOSPHO2 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PHOSPHO2
Gene ID: 493911
Uniprot ID: PHOP2_HUMAN
Immunogen Region: 1-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human PHOSPHO2 (NP_001008489.1).
Immunogen Sequence: MKILLVFDFDNTIIDDNSDT WIVQCAPNKKLPIELRDSYR KGFWTEFMGRVFKYLGDKGV REHEMKRAVTSLPFTPGMVE LFNFIRKNKDKFDCIIISDS
Function Phosphatase that has high activity toward pyridoxal 5'-phosphate (PLP). Also active at much lower level toward pyrophosphate, phosphoethanolamine (PEA), phosphocholine (PCho), phospho-l-tyrosine, fructose-6-phosphate, p-nitrophenyl phosphate, and h-glycerophosphate.
Protein Name Pyridoxal Phosphate Phosphatase Phospho2
Alternative Antibody Names Anti-Pyridoxal Phosphate Phosphatase Phospho2 antibody
Anti-PHOSPHO2 antibody

Information sourced from Uniprot.org

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