• Western blot analysis of extracts of Jurkat cells, using Pan Phospho-Tyrosine pAb (STJ11101040) at 1:1000 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit . Exposure time: 1s.
  • Immunofluorescence analysis of C6 cells using Pan Phospho-Tyrosine (STJ11101040) at dilution of 1:100. Blue: DAPI for nuclear staining. C6 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.
  • Immunofluorescence analysis of NIH/3T3 cells using Pan Phospho-Tyrosine (STJ11101040) at dilution of 1:100. Blue: DAPI for nuclear staining. NIH/3T3 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.
  • Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Pan Phospho-Tyrosine antibody (STJ11101040). Western blot was performed from the immunoprecipitate using Pan Phospho-Tyrosine antibody (STJ11101040) at a dilition of 1:1000. Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.

Anti-Phospho-Pan Phospho-Tyrosine-antibody (STJ11101040)

SKU:
STJ11101040

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB, IF, IP
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-Pan Phospho-Tyrosine is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Immunogen: A synthetic peptide corresponding to a sequence containing phosphorylated Y.
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance