• Western blot analysis of extracts of NIH/3T3 cells, using pan-Phospho-Serine antibody (STJ11101066) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Pan-Phospho-Serine antibody (STJ11101066)

SKU:
STJ11101066

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Host: Rabbit
Applications: WB
Reactivity: ALL
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-Pan-Phospho-Serine is suitable for use in Western Blot.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Immunogen: A synthetic peptide corresponding to a sequence containing phosphorylated Serine.
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance