• Immunohistochemistry analysis of paraffin-embedded human brain, using MDC1 (Phospho-Ser513) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MDC1-Ser513 antibody (479-528 aa) (STJ91260)

SKU:
STJ91260

Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Mediator of DNA damage checkpoint protein 1-Ser513 (479-528 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MDC1
Gene ID: 9656
Uniprot ID: MDC1_HUMAN
Immunogen Region: 479-528 aa
Specificity: Phospho-MDC1 (S513) Polyclonal Antibody detects endogenous levels of MDC1 protein only when phosphorylated at S513.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MDC1 around the phosphorylation site of Ser513 at the amino acid range 479-528
Post Translational Modifications Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint. Phosphorylation at Thr-4 by ATM stabilizes and enhances homodimerization via the FHA domain. Sumoylation at Lys-1840 by PIAS4 following DNA damage promotes ubiquitin-mediated degradation. Ubiquitinated by RNF4, leading to proteasomal degradation.undergoes 'Lys-48'-linked polyubiquitination.
Function Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
Protein Name Mediator Of Dna Damage Checkpoint Protein 1
Nuclear Factor With Brct Domains 1
Database Links Reactome: R-HSA-3108214
Reactome: R-HSA-5693565
Reactome: R-HSA-5693571
Reactome: R-HSA-5693607
Reactome: R-HSA-6796648
Reactome: R-HSA-69473
Cellular Localisation Nucleus
Chromosome
Associated With Chromatin
Relocalizes To Discrete Nuclear Foci Following Dna Damage
This Requires 'Ser-139' Phosphorylation Of H2ax
Colocalizes With Aptx At Sites Of Dna Double-Strand Breaks
Alternative Antibody Names Anti-Mediator Of Dna Damage Checkpoint Protein 1 antibody
Anti-Nuclear Factor With Brct Domains 1 antibody
Anti-MDC1 antibody
Anti-KIAA0170 antibody
Anti-NFBD1 antibody

Information sourced from Uniprot.org

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