| Host: | Rabbit |
| Applications: | ELISA/IHC/IP/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-JAK3 (750-800) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | FITC |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.5 µg/µl |
| Dilution Range: | WB: 1:500 ELISA: 1:10, 000 IP: 1:150 IHC: 1:50-1:100 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | JAK3 |
| Gene ID: | 3718 |
| Uniprot ID: | JAK3_HUMAN |
| Immunogen Region: | 750-800 |
| Immunogen: | Synthetic peptide taken within amino acid region 750-800 on human JAK3 protein. Phosphorylated tyrosine 785 residue. |
| Tissue Specificity | In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins. |
| Post Translational Modifications | Tyrosine phosphorylated in response to IL-2 and IL-4. Dephosphorylation of Tyr-980 and Tyr-981 by PTPN2 negatively regulates cytokine-mediated signaling (Probable). |
| Function | Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A and STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion. |
| Protein Name | Tyrosine-Protein Kinase Jak3 Janus Kinase 3 Jak-3 Leukocyte Janus Kinase L-Jak |
| Database Links | Reactome: R-HSA-1266695 Reactome: R-HSA-201556 Reactome: R-HSA-5673001 Reactome: R-HSA-6785807 Reactome: R-HSA-8854691 Reactome: R-HSA-8983432 Reactome: R-HSA-8985947 Reactome: R-HSA-9020558 Reactome: R-HSA-9020958 Reactome: R-HSA-912526 Reactome: R-HSA-9679191 |
| Cellular Localisation | Endomembrane System Peripheral Membrane Protein Cytoplasm |
| Alternative Antibody Names | Anti-Tyrosine-Protein Kinase Jak3 antibody Anti-Janus Kinase 3 antibody Anti-Jak-3 antibody Anti-Leukocyte Janus Kinase antibody Anti-L-Jak antibody Anti-JAK3 antibody |
Information sourced from Uniprot.org