• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ITGB4 (Phospho-Tyr1510) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from HepG2 cells treated with Na2VO3 0.3nM 40', using ITGB4 (Phospho-Tyr1510) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of AD293 using Phospho-Integrin Beta 4 (Y1510) Polyclonal Antibody. Antibody was diluted at 1:500

Anti-Phospho-ITGB4-Tyr1510 antibody (1481-1530 aa) (STJ90915)

SKU:
STJ90915

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Integrin beta-4-Tyr1510 (1481-1530 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITGB4
Gene ID: 3691
Uniprot ID: ITB4_HUMAN
Immunogen Region: 1481-1530 aa
Specificity: Phospho-Integrin Beta 4 (Y1510) Polyclonal Antibody detects endogenous levels of Integrin Beta 4 protein only when phosphorylated at Y1510.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ITGB4 around the phosphorylation site of Tyr1510 at the amino acid range 1481-1530
Post Translational Modifications Palmitoylated by DHHC3 at several cysteines of the membrane-proximal region, enhancing stability and cell surface expression. Palmitoylation also promotes secondary association with tertaspanins.
Function Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling.
Protein Name Integrin Beta-4
Gp150
Cd Antigen Cd104
Database Links Reactome: R-HSA-2022090
Reactome: R-HSA-3000157
Reactome: R-HSA-3000170
Reactome: R-HSA-446107
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Lipid-Anchor
Cell Junction
Hemidesmosome
Colocalizes With Dst At The Leading Edge Of Migrating Keratinocytes
Alternative Antibody Names Anti-Integrin Beta-4 antibody
Anti-Gp150 antibody
Anti-Cd Antigen Cd104 antibody
Anti-ITGB4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance