• Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-ITGA4-Ser1027 antibody (983-1032 aa) (STJ91315)

SKU:
STJ91315

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Integrin alpha-4-Ser1027 (983-1032 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITGA4
Gene ID: 3676
Uniprot ID: ITA4_HUMAN
Immunogen Region: 983-1032 aa
Specificity: Phospho-Integrin Alpha 4 (S1027) Polyclonal Antibody detects endogenous levels of Integrin Alpha 4 protein only when phosphorylated at S1027.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Integrin alpha4 around the phosphorylation site of Ser1027 at the amino acid range 983-1032
Function Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1.
Protein Name Integrin Alpha-4
Cd49 Antigen-Like Family Member D
Integrin Alpha-Iv
Vla-4 Subunit Alpha
Cd Antigen Cd49d
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-202733
Reactome: R-HSA-216083
Reactome: R-HSA-8949275
Reactome: R-HSA-9679191
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Integrin Alpha-4 antibody
Anti-Cd49 Antigen-Like Family Member D antibody
Anti-Integrin Alpha-Iv antibody
Anti-Vla-4 Subunit Alpha antibody
Anti-Cd Antigen Cd49d antibody
Anti-ITGA4 antibody
Anti-CD49D antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance