| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-NF-kappa-B essential modulator-Ser31 (16-65 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | IKBKG |
| Gene ID: | 8517 |
| Uniprot ID: | NEMO_HUMAN |
| Immunogen Region: | 16-65 aa |
| Specificity: | Phospho-IKK Gamma (S31) Polyclonal Antibody detects endogenous levels of IKK Gamma protein only when phosphorylated at S31. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human IKK-gamma around the phosphorylation site of Ser31 at the amino acid range 16-65 |
| Post Translational Modifications | Phosphorylation at Ser-68 attenuates aminoterminal homodimerization. Polyubiquitinated on Lys-285 via 'Lys-63'-linked ubiquitin.the ubiquitination is mediated downstream of NOD2 and RIPK2 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Polyubiquitinated on Lys-285 and Lys-399 through 'Lys-63'-linked ubiquitin.the ubiquitination is mediated by BCL10, MALT1 and TRAF6 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Monoubiquitinated on Lys-277 and Lys-309.promotes nuclear export. Polyubiquitinated through 'Lys-27' by TRIM23.involved in antiviral innate and inflammatory responses. Linear polyubiquitinated on Lys-111, Lys-143, Lys-226, Lys-246, Lys-264, Lys-277, Lys-285, Lys-292, Lys-302, Lys-309 and Lys-326.the head-to-tail polyubiquitination is mediated by the LUBAC complex and plays a key role in NF-kappa-B activation. Deubiquitinated by USP10 in a TANK-dependent and -independent manner, leading to the negative regulation of NF-kappa-B signaling upon DNA damage. Ubiquitinated at Lys-326 by MARCHF2 following bacterial and viral infection which leads to its degradation. Polyubiquitinated via 'Lys-29'-linked ubiquitin.leading to lysosomal degradation. Sumoylated on Lys-277 and Lys-309 with SUMO1.the modification results in phosphorylation of Ser-85 by ATM leading to a replacement of the sumoylation by mono-ubiquitination on these residues. Neddylated by TRIM40, resulting in stabilization of NFKBIA and down-regulation of NF-kappa-B activity. (Microbial infection) Cleaved by hepatitis A virus (HAV) protease 3C allowing the virus to disrupt the host innate immune signaling. (Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1 on both 'Lys-48' and 'Lys-63'-linked ubiquitin chains.leading to NF-kappa-B signaling inhibition. (Microbial infection) Polyubiquitinated on Lys-309 and Lys-321 via 'Lys-27'-linked ubiquitin by Shigella flexneri E3 ubiquitin-protein ligase ipah9.8, leading to its degradation by the proteasome. (Microbial infection) Polyubiquitination through 'Lys-63' is interrupted by interaction with SARS coronavirus-2/SARS-CoV-2 virus protein ORF9B which inhibits the NF-kappa-B pathway. |
| Function | Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin plays a key role in IKK activation by multiple signaling receptor pathways. Can recognize and bind both 'Lys-63'-linked and linear polyubiquitin upon cell stimulation, with a much higher affinity for linear polyubiquitin. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response.this function requires 'Lys-27'-linked polyubiquitination. (Microbial infection) Also considered to be a mediator for HTLV-1 Tax oncoprotein activation of NF-kappa-B. |
| Protein Name | Nf-Kappa-B Essential Modulator Nemo Fip-3 Ikb Kinase-Associated Protein 1 Ikkap1 Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Gamma I-Kappa-B Kinase Subunit Gamma Ikk-Gamma Ikkg Ikb Kinase Subunit Gamma Nf-Kappa-B Essential Modifier |
| Database Links | Reactome: R-HSA-1169091 Reactome: R-HSA-1236974 Reactome: R-HSA-168638 Reactome: R-HSA-168927 Reactome: R-HSA-1810476 Reactome: R-HSA-202424 Reactome: R-HSA-2871837 Reactome: R-HSA-445989 Reactome: R-HSA-450302 Reactome: R-HSA-450321 Reactome: R-HSA-4755510 Reactome: R-HSA-5357905 Reactome: R-HSA-5357956 Reactome: R-HSA-5602636 Reactome: R-HSA-5603027 Reactome: R-HSA-5603029 Reactome: R-HSA-5607764 Reactome: R-HSA-5684264 Reactome: R-HSA-5689880 Reactome: R-HSA-5689896 Reactome: R-HSA-9020702 Reactome: R-HSA-933542 Reactome: R-HSA-933543 Reactome: R-HSA-937039 Reactome: R-HSA-937041 Reactome: R-HSA-9705671 Reactome: R-HSA-975144 Reactome: R-HSA-9758274 Reactome: R-HSA-9833482 Reactome: R-HSA-9860276 |
| Cellular Localisation | Cytoplasm Nucleus Sumoylated Nemo Accumulates In The Nucleus In Response To Genotoxic Stress |
| Alternative Antibody Names | Anti-Nf-Kappa-B Essential Modulator antibody Anti-Nemo antibody Anti-Fip-3 antibody Anti-Ikb Kinase-Associated Protein 1 antibody Anti-Ikkap1 antibody Anti-Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Gamma antibody Anti-I-Kappa-B Kinase Subunit Gamma antibody Anti-Ikk-Gamma antibody Anti-Ikkg antibody Anti-Ikb Kinase Subunit Gamma antibody Anti-Nf-Kappa-B Essential Modifier antibody Anti-IKBKG antibody Anti-FIP3 antibody Anti-NEMO antibody |
Information sourced from Uniprot.org