• Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30', using DRP-2 (Phospho-Thr514) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of HeLa cells, using DRP-2 (Phospho-Thr514) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain, using DRP-2 (Phospho-Thr514) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of 3T3 cells using Phospho-CRMP-2 (T514) Polyclonal Antibody

Anti-Phospho-DPYSL2-Thr514 antibody (480-529 aa) (STJ91107)

SKU:
STJ91107

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Dihydropyrimidinase-related protein 2-Thr514 (480-529 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DPYSL2
Gene ID: 1808
Uniprot ID: DPYL2_HUMAN
Immunogen Region: 480-529 aa
Specificity: Phospho-CRMP-2 (T514) Polyclonal Antibody detects endogenous levels of CRMP-2 protein only when phosphorylated at T514.
Immunogen: The antiserum was produced against synthesized peptide derived from the human DRP-2 around the phosphorylation site of Thr514 at the amino acid range 480-529
Post Translational Modifications 3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509. Phosphorylation at Thr-514 by GSK3B abolishes tubulin-binding leading to destabilization of microtubule assembly in axons and neurodegeneration. Phosphorylation by DYRK2 at Ser-522 is required for subsequent phosphorylation by GSK3B.
Function Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis.
Protein Name Dihydropyrimidinase-Related Protein 2
Drp-2
Collapsin Response Mediator Protein 2
Crmp-2
N2a3
Unc-33-Like Phosphoprotein 2
Ulip-2
Database Links Reactome: R-HSA-399956
Reactome: R-HSA-437239
Cellular Localisation Cytoplasm
Cytosol
Cytoskeleton
Membrane
Tightly But Non-Covalently Associated With Membranes
Alternative Antibody Names Anti-Dihydropyrimidinase-Related Protein 2 antibody
Anti-Drp-2 antibody
Anti-Collapsin Response Mediator Protein 2 antibody
Anti-Crmp-2 antibody
Anti-N2a3 antibody
Anti-Unc-33-Like Phosphoprotein 2 antibody
Anti-Ulip-2 antibody
Anti-DPYSL2 antibody
Anti-CRMP2 antibody
Anti-ULIP2 antibody

Information sourced from Uniprot.org

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