• Western blot analysis of extracts of various cell lines, using Phospho-Cyclin D1-T286 polyclonal antibody (STJ29340) at 1:1000 dilution. C6 cells were treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-CCND1-T286 antibody (STJ29340)

SKU:
STJ29340

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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-CCND1-T286 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCND1
Gene ID: 595
Uniprot ID: CCND1_HUMAN
Immunogen: A synthetic phosphorylated peptide around T286 of human Cyclin D1 (NP_444284.1).
Immunogen Sequence: ACTPT
Post Translational Modifications Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation by the DCX(AMBRA1) complex. It also plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex following DNA damage. Ubiquitinated at Lys-269 by the DCX(AMBRA1) complex during the transition from G1 to S cell phase, leading to its degradation: ubiquitination is dependent on Thr-286 phosphorylation. The DCX(AMBRA1) complex represents the major regulator of CCND1 stability during the G1/S transition. Also ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB. Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. SCF-type ubiquitination is dependent on Thr-286 phosphorylation. Ubiquitinated also by UHRF2 apparently in a phosphorylation-independent manner. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2.leading to its stabilization.
Function Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also a substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
Protein Name G1/S-Specific Cyclin-D1
B-Cell Lymphoma 1 Protein
Bcl-1
Bcl-1 Oncogene
Prad1 Oncogene
Database Links Reactome: R-HSA-187577
Reactome: R-HSA-1912408
Reactome: R-HSA-3214858
Reactome: R-HSA-6785807
Reactome: R-HSA-69231
Reactome: R-HSA-75815
Reactome: R-HSA-8849470
Reactome: R-HSA-8853884
Reactome: R-HSA-8878166
Reactome: R-HSA-8934593
Reactome: R-HSA-8951430
Reactome: R-HSA-8951936
Reactome: R-HSA-9018519
Reactome: R-HSA-9634638
Reactome: R-HSA-9661069
Reactome: R-HSA-9754119
Cellular Localisation Nucleus
Cytoplasm
Nucleus Membrane
Cyclin D-Cdk4 Complexes Accumulate At The Nuclear Membrane And Are Then Translocated To The Nucleus Through Interaction With Kip/Cip Family Members
Alternative Antibody Names Anti-G1/S-Specific Cyclin-D1 antibody
Anti-B-Cell Lymphoma 1 Protein antibody
Anti-Bcl-1 antibody
Anti-Bcl-1 Oncogene antibody
Anti-Prad1 Oncogene antibody
Anti-CCND1 antibody
Anti-BCL1 antibody
Anti-PRAD1 antibody

Information sourced from Uniprot.org

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