| Host: | Rabbit |
| Applications: | IHC-P |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit monoclonal antibody anti-PHH3 (1-100aa) is suitable for use in Immunohistochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | ZR285 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin |
| Purification: | Affinity purified |
| Dilution Range: | 1:100-200 |
| Storage Instruction: | Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C. |
| Immunogen Region: | 1-100aa |
| Specificity: | Positive control: Tonsil |
| Immunogen: | Synthetic peptide corresponding to phosphohistone H3 residue within aa 1-100 |
| Background | Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei is from 0.0 to 6.6% (median value 0.8%). Increased expression of PHH3was significantly associated with tumor thickness (p = 0.031) , presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027) , but not with Clark s level of invasion. High levels of PHH3 is associated with increased mitotic count (p = 0.003) and high Ki-67 expression (p = 0.002). For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc., PHH3 mAb is helpful for tumor pathological classification and prognosis. |
Information sourced from Uniprot.org