• Western blot analysis of extracts of various cell lines, using PHF19 antibody (STJ110368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of A-549 cells using PHF19 Rabbit polyclonal antibody (STJ110368). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.
  • Immunofluorescence analysis of A-549 cells using PHF19 antibody (STJ110368).
  • Western blot analysis of various lysates using PHF19 Rabbit polyclonal antibody (STJ110368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PHF19 antibody (1-207) (STJ110368)

SKU:
STJ110368

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PHF19 (1-207) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PHF19
Gene ID: 26147
Uniprot ID: PHF19_HUMAN
Immunogen Region: 1-207
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human PHF19 (NP_001009936.1).
Immunogen Sequence: MENRALDPGTRDSYGATSHL PNKGALAKVKNNFKDLMSKL TEGQYVLCRWTDGLYYLGKI KRVSSSKQSCLVTFEDNSKY WVLWKDIQHAGVPGEEPKCN ICLGKTSGPLNEILICGKCG LGYHQQCHIPIAGSADQPLL TPWFCRRCIFALAVRVSLPS SPVPASPASSSGADQRLPSQ SLSSKQKGHTWALETDSASA TVLGQDL
Tissue Specificity Isoform 1 is expressed in thymus, heart, lung and kidney. Isoform 2 is predominantly expressed in placenta, skeletal muscle and kidney, whereas isoform 1 is predominantly expressed in liver and peripheral blood leukocytes. Overexpressed in many types of cancers, including colon, skin, lung, rectal, cervical, uterus, liver cancers, in cell lines derived from different stages of melanoma and in glioma cell lines.
Function Polycomb group (PcG) protein that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex, thus enhancing PRC2 H3K27me3 methylation activity. Probably involved in the transition from an active state to a repressed state in embryonic stem cells: acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting H3K36me3 histone demethylases RIOX1 or KDM2B, leading to demethylation of H3K36 and recruitment of the PRC2 complex that mediates H3K27me3 methylation, followed by de novo silencing. Recruits the PRC2 complex to CpG islands and contributes to embryonic stem cell self-renewal. Also binds histone H3 dimethylated at 'Lys-36' (H3K36me2). Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter.
Protein Name Phd Finger Protein 19
Polycomb-Like Protein 3
Hpcl3
Database Links Reactome: R-HSA-212300
Cellular Localisation Nucleus
Localizes To Chromatin As Part Of The Prc2 Complex
Alternative Antibody Names Anti-Phd Finger Protein 19 antibody
Anti-Polycomb-Like Protein 3 antibody
Anti-Hpcl3 antibody
Anti-PHF19 antibody
Anti-PCL3 antibody

Information sourced from Uniprot.org

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