• Immunohistochemistry analysis of paraffin-embedded human placenta using ERp57 Rabbit monoclonal antibody (STJ11102061) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ERp57 Rabbit monoclonal antibody (STJ11102061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PDIA3 antibody (100-200) [S1MR] (STJ11102061)

SKU:
STJ11102061

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ERp57 (100-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDIA3
Gene ID: 2923
Uniprot ID: PDIA3_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human ERp57 (P30101).
Immunogen Sequence: YPTLKIFRDGEEAGAYDGPR TADGIVSHLKKQAGPASVPL RTEEEFKKFISDKDASIVGF FDDSFSEAHSEFLKAASNLR DNYRFAHTNVESLVNEYDDN G
Tissue Specificity Detected in the flagellum and head region of spermatozoa (at protein level). Expressed in liver, stomach and colon (at protein level). Expressed in gastric parietal cells and chief cells (at protein level).
Post Translational Modifications Within the major histocompatibility complex class I (MHC I) peptide loading complex forms reversible disulfide-linked heterodimers with TAPBP as part of its protein folding chaperone activity. This is essential to assist the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Phosphorylated.
Function Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone. Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it functions as an essential folding chaperone for TAPBP. Through TAPBP, assists the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Therefore, plays a crucial role in the presentation of antigens to cytotoxic T cells in adaptive immunity.
Protein Name Protein Disulfide-Isomerase A3
58 Kda Glucose-Regulated Protein
58 Kda Microsomal Protein
P58
Disulfide Isomerase Er-60
Endoplasmic Reticulum Resident Protein 57
Er Protein 57
Erp57
Endoplasmic Reticulum Resident Protein 60
Er Protein 60
Erp60
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-901042
Reactome: R-HSA-983170
Cellular Localisation Endoplasmic Reticulum
Endoplasmic Reticulum Lumen
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Protein Disulfide-Isomerase A3 antibody
Anti-58 Kda Glucose-Regulated Protein antibody
Anti-58 Kda Microsomal Protein antibody
Anti-P58 antibody
Anti-Disulfide Isomerase Er-60 antibody
Anti-Endoplasmic Reticulum Resident Protein 57 antibody
Anti-Er Protein 57 antibody
Anti-Erp57 antibody
Anti-Endoplasmic Reticulum Resident Protein 60 antibody
Anti-Er Protein 60 antibody
Anti-Erp60 antibody
Anti-PDIA3 antibody
Anti-ERP57 antibody
Anti-ERP60 antibody
Anti-GRP58 antibody

Information sourced from Uniprot.org

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