| Host: |
Rabbit |
| Applications: |
CM/ELISA/ICC/IF/IHC/IP/WB |
| Reactivity: |
Human/Moose/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PDE4A (C-Term) is suitable for use in Confocal Microscopy, ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Purification: |
Affinity Purified |
| Concentration: |
0.5 µg/µl |
| Dilution Range: |
WB: 1:1, 000-1:2, 500DB: 1:10, 000ELISA: 1:10, 000IP: 1:250IHC: 1:100-1:400ICC: 1:100-1:400IF: 1:100-1:400CFM: 1:100-1:400 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
PDE4A |
| Gene ID: |
5141 |
| Uniprot ID: |
PDE4A_HUMAN |
| Immunogen Region: |
C-Term |
| Specificity: |
This antibody labels all known PDE4A variants including PDE4A1 (66 kDa) , PDE4A5 (109 kDa) , PDE4A8 (106 kDa) , AND PDE4Ax (10 kDa) and a 76 kDa testis specific A variant. |
| Immunogen: |
Synthetic cyclic peptide corresponding to the C-terminal region of PDE4A. Common to all PDE4A proteins. |
| Tissue Specificity | Isoform 1: Expressed in lymphoid cell subsets including CD8-positive T cells and T-helper 2 cells. Expressed in dendritic cells. Isoform 2: Highly expressed in liver, stomach, testis, thyroid and adrenal glands and at a lower extent in placenta, kidney, pancreas, ovary, uterus and skin. Expressed in myeloid cell subsets including dendritic cells, monocytes, macrophages, eosinophils and mast cells. Expressed in natural killer cells. Expressed in bronchial smooth muscle. Isoform 6: Expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7: Expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Found in specific neuronal subpopulations including cortical pyramidal neurons, horn neurons in the spinal cord and Purkinje cells in cerebellum (at protein level). |
| Post Translational Modifications | Isoform 1: Proteolytically cleaved by CASP3. Isoform 2: Phosphorylated at Ser-119 by PKA. |
| Function | Hydrolyzes the second messenger 3',5'-cyclic AMP (cAMP), which is a key regulator of many important physiological processes. Isoform 1: Efficiently hydrolyzes cAMP. Isoform 2: Efficiently hydrolyzes cAMP. Isoform 3: Efficiently hydrolyzes cAMP. The phosphodiesterase activity is not affected by calcium, calmodulin or cyclic GMP (cGMP) levels. Does not hydrolyze cGMP. Isoform 4: Efficiently hydrolyzes cAMP. Isoform 6: Efficiently hydrolyzes cAMP. Isoform 7: Efficiently hydrolyzes cAMP. |
| Protein Name | Camp-Specific 3' -5'-Cyclic Phosphodiesterase 4aDpde2Pde46 |
| Database Links | Reactome: R-HSA-180024Reactome: R-HSA-418555 |
| Cellular Localisation | Isoform 1: CytoplasmPerinuclear RegionIsoform 2: CytoplasmCell ProjectionRuffle MembraneIsoform 3: CytoplasmCytosolIsoform 4: MembranePeripheral Membrane ProteinIsoform 4 Has Propensity For Association With MembranesIsoform 6: CytoplasmIsoform 7: CytoplasmMembranePredominantly Cytosolic |
| Alternative Antibody Names | Anti-Camp-Specific 3' -5'-Cyclic Phosphodiesterase 4a antibodyAnti-Dpde2 antibodyAnti-Pde46 antibodyAnti-PDE4A antibodyAnti-DPDE2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
