• Immunoprecipitation analysis of 200 Mu g extracts from MCF7 cells using 3 Mu g Pan-Akt Rabbit monoclonal antibody (STJ11102945). Western blot was performed from the immunoprecipitate using Pan-Akt Rabbit monoclonal antibody (STJ11102945) at a dilution of 1:1000.
  • Confocal immunofluorescence analysis of C6 cells using Pan-Akt Rabbit monoclonal antibody (STJ11102945, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of paraffin-embedded Human testis tissue using Pan-Akt Rabbit monoclonal antibody (STJ11102945, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.
  • Confocal immunofluorescence analysis of C2C12 cells using Pan-Akt Rabbit monoclonal antibody (STJ11102945, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry analysis of Pan-Akt in paraffin-embedded rat colon tissue using Pan-Akt Rabbit monoclonal antibody (STJ11102945) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Pan-Akt in paraffin-embedded mouse testis tissue using Pan-Akt Rabbit monoclonal antibody (STJ11102945) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Pan-Akt in paraffin-embedded mouse intestin tissue using Pan-Akt Rabbit monoclonal antibody (STJ11102945) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Pan-Akt in paraffin-embedded human colon carcinoma tissue using Pan-Akt Rabbit monoclonal antibody (STJ11102945) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Pan-Akt in paraffin-embedded human cervix cancer tissue using Pan-Akt Rabbit monoclonal antibody (STJ11102945) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using Pan-Akt Rabbit monoclonal antibody (STJ11102945) at1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Pan-AKT antibody (1-123) [S5MR] (STJ11102945)

SKU:
STJ11102945

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Pan-AKT (1-123) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:10000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-123
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-123 of human Pan-Akt (NP_005154.2).
Immunogen Sequence: MSDVAIVKEGWLHKRGEYIK TWRPRYFLLKNDGTFIGYKE RPQDVDQREAPLNNFSVAQC QLMKTERPRPNTFIIRCLQW TTVIERTFHVETPEEREEWT TAIQTVADGLKKQEEEEMDF RSG
Background Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

Information sourced from Uniprot.org

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