• Immunofluorescence analysis of NIH-3T3 cells using PALLD Rabbit pAb (STJ114240) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using PALLD Rabbit pAb (STJ114240) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat ovary using PALLD Rabbit pAb (STJ114240) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using PALLD Rabbit pAb (STJ114240) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using PALLD antibody (STJ114240) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

Anti-PALLD antibody (STJ114240)

SKU:
STJ114240

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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-PALLD antibody is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
Uniprot ID: PALLD_HUMAN
Gene ID: 23022
Gene Symbol: PALLD
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 383-672 of human PALLD (NP_001159582.1).
Tissue Specificity Detected in both muscle and non-muscle tissues. High expression in prostate, ovary, colon, and kidney. Not detected in spleen, skeletal muscle, lung and peripheral blood lymphocytes (at protein level). Protein is overexpressed in FA6, HPAF, IMIM-PC2, SUIT-2 and PancTu-II sporadic pancreatic cancer cell lines.
Post Translational Modifications Phosphorylated predominantly on serines and, to a lesser extent, on tyrosines. Phosphorylation at Ser-1118 by PKB/AKT1 modulates cytoskeletal organization and cell motility.
Function Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific subcellular foci.
Protein Name Palladin
Sih002
Sarcoma Antigen Ny-Sar-77
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Junction
Focal Adhesion
Myofibril
Sarcomere
Z Line
Cell Projection
Ruffle
Podosome
Lamellipodium
Axon
Growth Cone
Localizes To Stress Fibers And Z Lines
Preferentially Expressed In The Excitatory Presynaptic Terminals
Alternative Antibody Names Anti-Palladin antibody
Anti-Sih002 antibody
Anti-Sarcoma Antigen Ny-Sar-77 antibody
Anti-PALLD antibody
Anti-KIAA0992 antibody
Anti-CGI-151 antibody

Information sourced from Uniprot.org

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