• Immunofluorescence analysis of NIH/3T3 cells using OTULIN Rabbit polyclonal antibody (STJ11103341) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of MCF7 cells, using OTULIN antibody (STJ11103341) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of Rat liver, using OTULIN antibody (STJ11103341) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-OTULIN antibody (50-352) (STJ11103341)

SKU:
STJ11103341

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-OTULIN (50-352) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OTULIN
Gene ID: 90268
Uniprot ID: OTUL_HUMAN
Immunogen Region: 50-352
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-352 of human OTULIN (NP_612357.4).
Immunogen Sequence: EHEEDMYRAADEIEKEKELL IHERGASEPRLSVAPEMDIM DYCKKEWRGNTQKATCMKMG YEEVSQKFTSIRRVRGDNYC ALRATLFQAMSQAVGLPPWL QDPELMLLPEKLISKYNWIK QWKLGLKFDGKNEDLVDKIK ESLTLLRKKWAGLAEMRTAE ARQIACDELFTNEAEEYSLY EAVKFLMLNRAIELYNDKEK GKEVPFFSVLLFARDTSNDP GQLLRNHLNQVGHTGGLEQ
Post Translational Modifications Ubiquitinated. Acetylated. Phosphorylated. Phosphorylation at Tyr-56 prevents interaction with RNF31.dephosphorylation promotes interaction with RNF31 and the LUBAC complex.
Function Deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response. Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex. Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex. OTULIN function is mainly restricted to homeostasis of the LUBAC complex: acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex. Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis. In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation. Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent pro-inflammatory signaling.
Protein Name Ubiquitin Thioesterase Otulin
Deubiquitinating Enzyme Otulin
Otu Domain-Containing Deubiquitinase With Linear Linkage Specificity
Ubiquitin Thioesterase Gumby
Database Links Reactome: R-HSA-5357905
Reactome: R-HSA-8866652
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Ubiquitin Thioesterase Otulin antibody
Anti-Deubiquitinating Enzyme Otulin antibody
Anti-Otu Domain-Containing Deubiquitinase With Linear Linkage Specificity antibody
Anti-Ubiquitin Thioesterase Gumby antibody
Anti-OTULIN antibody
Anti-FAM105B antibody

Information sourced from Uniprot.org

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