• Western blot analysis of extracts of various cell lines, using NUP210 antibody (STJ119093) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of L929 cells using NUP210 antibody (STJ119093) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using NUP210 antibody (STJ119093) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-NUP210 antibody (680-880) (STJ119093)

SKU:
STJ119093

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NUP210 (680-880) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUP210
Gene ID: 23225
Uniprot ID: PO210_HUMAN
Immunogen Region: 680-880
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 680-880 of human NUP210 (NP_079199.2).
Immunogen Sequence: QNVTAEDTDSIGLALFAPHS SRNYQQHWILVTCQALGEQV IALSVGNKPSLTNPFPAVEP AVVKFVCAPPSRLTLAPVYT SPQLDMSCPLLQQNKQVVPV SSHRNPRLDLAAYDQEGRRF DNFSSLSIQWESTRPVLASI EPELPMQLVSQDDESGQKKL HGLQAILVHEASGTTAITAT ATGYQESHLSSARTKQPHDP L
Tissue Specificity Ubiquitous expression, with highest levels in lung, liver, pancreas, testis, and ovary, intermediate levels in brain, kidney, and spleen, and lowest levels in heart and skeletal muscle.
Post Translational Modifications N-glycosylated, but not all potential glycosylation sites may be used. Contains high-mannose type oligosaccharides. Phosphorylated at Ser-1881 in mitosis specifically.not phosphorylated in interphase.
Function Nucleoporin essential for nuclear pore assembly and fusion, nuclear pore spacing, as well as structural integrity.
Protein Name Nuclear Pore Membrane Glycoprotein 210
Nuclear Pore Protein Gp210
Nuclear Envelope Pore Membrane Protein Pom 210
Pom210
Nucleoporin Nup210
Pore Membrane Protein Of 210 Kda
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-159227
Reactome: R-HSA-159230
Reactome: R-HSA-159231
Reactome: R-HSA-159236
Reactome: R-HSA-165054
Reactome: R-HSA-168271
Reactome: R-HSA-168276
Reactome: R-HSA-168325
Reactome: R-HSA-168333
Reactome: R-HSA-170822
Reactome: R-HSA-180746
Reactome: R-HSA-180910
Reactome: R-HSA-191859
Reactome: R-HSA-3108214
Reactome: R-HSA-3232142
Reactome: R-HSA-3301854
Reactome: R-HSA-3371453
Reactome: R-HSA-4085377
Reactome: R-HSA-4551638
Reactome: R-HSA-4570464
Reactome: R-HSA-4615885
Reactome: R-HSA-5578749
Reactome: R-HSA-5619107
Reactome: R-HSA-6784531
Reactome: R-HSA-9609690
Reactome: R-HSA-9610379
Reactome: R-HSA-9705671
Cellular Localisation Nucleus
Nuclear Pore Complex
Nucleus Membrane
Single-Pass Type I Membrane Protein
Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Nuclear Pore Membrane Glycoprotein 210 antibody
Anti-Nuclear Pore Protein Gp210 antibody
Anti-Nuclear Envelope Pore Membrane Protein Pom 210 antibody
Anti-Pom210 antibody
Anti-Nucleoporin Nup210 antibody
Anti-Pore Membrane Protein Of 210 Kda antibody
Anti-NUP210 antibody
Anti-KIAA0906 antibody
Anti-PSEC0245 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance