| Host: |
Rabbit |
| Applications: |
WB/IHC-P/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NSUN2 |
| Gene ID: |
54888 |
| Uniprot ID: |
NSUN2_HUMAN |
| Immunogen Region: |
617-767 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 617-708 of human NSUN2 (NP_060225.4). |
| Immunogen Sequence: |
SRIITVSMEDVKILLTQENP FFRKLSSETYSQAKDLAKGS IVLKYEPDSANPDALQCPIV LCGWRGKASIRTFVPKNERL HYLRMMGLEVLG |
| Tissue Specificity | Expressed in adult and fetal brain and in lymphoblastoid cells. |
| Post Translational Modifications | Phosphorylated at Ser-139 by AURKB during mitosis, leading to abolish methyltransferase activity and the interaction with NPM1. |
| Function | RNA cytosine C(5)-methyltransferase that methylates cytosine to 5-methylcytosine (m5C) in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs). Involved in various processes, such as epidermal stem cell differentiation, testis differentiation and maternal to zygotic transition during early development: acts by increasing protein synthesis.cytosine C(5)-methylation promoting tRNA stability and preventing mRNA decay. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. tRNA methylation is required generation of RNA fragments derived from tRNAs (tRFs). Also mediates C(5)-methylation of mitochondrial tRNAs. Catalyzes cytosine C(5)-methylation of mRNAs, leading to stabilize them and prevent mRNA decay: mRNA stabilization involves YBX1 that specifically recognizes and binds m5C-modified transcripts. Cytosine C(5)-methylation of mRNAs also regulates mRNA export: methylated transcripts are specifically recognized by THOC4/ALYREF, which mediates mRNA nucleo-cytoplasmic shuttling. Also mediates cytosine C(5)-methylation of non-coding RNAs, such as vault RNAs (vtRNAs), promoting their processing into regulatory small RNAs. Cytosine C(5)-methylation of vtRNA VTRNA1.1 promotes its processing into small-vault RNA4 (svRNA4) and regulates epidermal differentiation. May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity. |
| Protein Name | Rna Cytosine C(5-Methyltransferase Nsun2Myc-Induced Sun Domain-Containing ProteinMisuNol1/Nop2/Sun Domain Family Member 2Substrate Of Aim1/Aurora Kinase BMrna Cytosine C(5-MethyltransferaseTrna Cytosine C(5-MethyltransferaseTrna Methyltransferase 4 HomologHtrm4 |
| Database Links | Reactome: R-HSA-6782315 |
| Cellular Localisation | NucleusNucleolusCytoplasmMitochondrionCytoskeletonSpindleSecretedExtracellular ExosomeConcentrated In The Nucleolus During Interphase And Translocates To The Spindle During Mitosis As An Rna-Protein Complex That Includes 18s Ribosomal RnaIn TestisLocalizes To The Chromatoid Body |
| Alternative Antibody Names | Anti-Rna Cytosine C(5-Methyltransferase Nsun2 antibodyAnti-Myc-Induced Sun Domain-Containing Protein antibodyAnti-Misu antibodyAnti-Nol1/Nop2/Sun Domain Family Member 2 antibodyAnti-Substrate Of Aim1/Aurora Kinase B antibodyAnti-Mrna Cytosine C(5-Methyltransferase antibodyAnti-Trna Cytosine C(5-Methyltransferase antibodyAnti-Trna Methyltransferase 4 Homolog antibodyAnti-Htrm4 antibodyAnti-NSUN2 antibodyAnti-SAKI antibodyAnti-TRM4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
